山西老陈醋对急性醉酒小鼠防醉及护肝作用研究

建立急性醉酒小鼠模型，将40只美国癌症研究所（ICR）小鼠随机分成空白对照组、模型组、低、高剂量醋组，并在灌胃4.0 h后，观察小鼠的的醉酒行为及小鼠肝组织病理学，并分别检测血清中乙醇含量及谷草转氨酶（ALT）、谷丙转氨酶（AST）活性和肝组织生化指标。结果表明，与模型组相比，高剂量醋组可显著延长醉酒时间至（44.00±9.35） min（P＜0.05）、醒酒时间极显著缩短至（121.25±12.71） min（P＜0.01）；血清中乙醇含量及谷丙转氨酶（ALT）、谷草转氨酶（AST）活性显著降低为（37.01±4.24） mg/100 mL、（12.83± 3.62） U/g、（14.22±1.56） U/g（P＜0.05）；肝组织中乙醇脱氢酶（ADH）活性极显著增加（P＜0.01）、乙醛脱氢酶（ALDH）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性显著增加（P＜0.05），且丙二醛（MDA）含量显著降低（P＜0.05）。食醋组肝脏病变程度减轻，切片显示空泡面积减少。结果表明山西老陈醋具有预防小鼠醉酒和护肝作用。

Anti-drunk and hepatoprotective effects of Shanxi aged vinegar on acute drunkenness mice

Mice model of acute intoxication was established, 40 American Institute of Cancer Researcch (ICR) mice were randomly divided into control group, model group, low-dose vinegar group and high-dose vinegar group. After 4.0 h of intragastric administration, the drunken behavior and liver histopathology of mice were observed, the alcohol content, the activities of alanine aminotransferase (ALT) and alanine aminotransferase (AST) in serum, and the biochemical indexes in liver tissue were measured respectively. The results showed that compared with model group, the drunkenness time of high-dose vinegar group was significantly prolonged to (44.00±9.35) min (P＜0.05) and the sobering time was reduced to (121.25±12.71) min (P＜0.01). The alcohol content and the activities of ALT and AST in serum were significantly decreased to (37.01±4.24) mg/100 ml, (12.83±3.62) U/g and (14.22±1.56) U/g (P＜0.05), the activity of alcohol dehydrogenase (ADH) in liver tissue was significantly increased (P＜0.01), the activities of acetaldehyde dehydrogenase (ALDH), catalase (CAT) and superoxide dismutase (SOD) in liver tissue were significantly increased (P＜0.05), and the malondialdehyde (MDA) content in liver tissue was significantly decreased (P＜0.05). The liver lesion degree was alleviated, and the hepatocyte vacuolar area was decreased in vinegar group. The results demonstrated that Shanxi aged vinegar could prevent mice drunkenness and protect liver.