| 产细菌素乳酸菌的选育及其抑菌特性的研究 |
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| 引用本文： | 吴荣荣,张良,王倩.产细菌素乳酸菌的选育及其抑菌特性的研究[J].中国酿造,2009(6). |
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| 作者姓名： | 吴荣荣 张良 王倩 |
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| 作者单位： | 衡水学院,生命科学学院,河北,衡水,053000 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，衡水学院院级项目 |
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| 摘 要： | 采用牛津杯琼脂扩散法，从实验室保存的乳酸菌中筛选到具有较高抑菌活性的菌株嗜酸乳杆菌A、戊糖片球菌M和戊糖片球菌T，它们产生的抑菌物质经排除酸、过氧化氢后，仍具有抑菌活性，然而经蛋白酶处理后其抑菌活性明显下降，确定其抑菌物质为细菌素，进一步的抑菌实验表明，3株菌所产细菌素具有较宽的抑菌谱，适宜用作生物型防腐剂。
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| 关 键 词： | 乳酸菌 细菌素 抑菌特性 |
Screening of bacteriocin-producing lactic acid bacteria and the characteristics of antibacterial compound |
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| WU Rongrong,ZHANG Liang,WANG Qian.Screening of bacteriocin-producing lactic acid bacteria and the characteristics of antibacterial compound[J].China Brewing,2009(6). |
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| Authors: | WU Rongrong ZHANG Liang WANG Qian |
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| Abstract: | Three strains, which showed obvious antibacterial activity, were isolated from lactic acid bacteria, including Lactobacillus.Acidophilus (A), Pediococcus pentosaceus(M)and Pediococcus pentosaceus(T)by the agar diffusion cup-plate method.The supernatants after eliminated organic acid and hydrogen peroxide still retained bacteriostatic activity.The effect of inhibition decreased after treatment with protease K, The above results indicated that the antibacterial substance was a kind of protein.The inhibitory spectrum from the above supernatants was broad.The bacteriocin produced by three strains was suitable for the biotype preservatives. |
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| Keywords: | lactic acid bacteria bacteriocin antibacterial characteristics |
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