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| 用DGGE技术构建白酒酿造微生物指纹图谱的初步研究 | |
| 引用本文： | 施思,张文学,邓宇,钟方达,胡锋.用DGGE技术构建白酒酿造微生物指纹图谱的初步研究[J].中国酿造,2010(1). |
| 作者姓名： | 施思 张文学 邓宇 钟方达 胡锋 |
| 作者单位： | 1. 四川大学,轻纺与食品学院,四川,成都,610065 2. 农业部沼气科学研究所,四川,成都,610041 3. 贵州茅台集团习酒责任有限公司,贵州,贵阳,564622 |
| 摘 要： | 利用PCR-DGGE技术及Quantity One软件分析对比了大曲、酒醅不同部位的微生物群落结构.通过从原始样品中直接提取DNA,并对全长16s rDNA及其V3区片断扩增,变性梯度凝胶电泳及软件分析等,发现不同样品之间的微生物群落存在一定联系及差异性.通过图谱与系统发育树的结合,能给不同样品赋予特定的分子标签.显示DGGE技术能为微生物菌落结构提供直观图谱,是研究自然传统发酵食品及其它天然微生态样品的先进方法. |
| 关 键 词： | 微生物 大曲 酒醅 |
Construction of microbial fingerprint in Chinese liquor production by DGGE technique |
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| SHI Si,ZHANG Wenxue,DENG Yu,ZHONG Fangda,HU Feng.Construction of microbial fingerprint in Chinese liquor production by DGGE technique[J].China Brewing,2010(1). | |
| Authors: | SHI Si ZHANG Wenxue DENG Yu ZHONG Fangda HU Feng |
| Affiliation: | SHI Si1,ZHANG Wenxue1,DENG Yu2,ZHONG Fangda3,HU Feng3 (1.College of Light Textile , Food Science,Sichuan University,Chengdu,610065,China,2.Biogas Institute of Ministry of Agriculture,Chengdu.610041,3.Xijiu Co.,Ltd.,of Maotai Group,Guizhou 564622,China) |
| Abstract: | Microbial community in different positions of Daqu and fermented grains were studied by PCR-DGGE and Quantity One software.By means of directly extracting DNA from samples,amplifying 16S rDNA and their V3 region by PCR,denaturing gradient gel electrophoresis (DGGE) and software analysis,it was found that among different samples,bacterial communities have connection and variability.The combination of DGGE graph and its phylogenetic tree could endue different samples with specified molecular labels.The DGGE t... |
| Keywords: | DGGE |
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