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混株发酵混合酒糟生产含酶蛋白饲料的研究
引用本文:陈曦,赵建国.混株发酵混合酒糟生产含酶蛋白饲料的研究[J].中国酿造,2006(5):30-33.
作者姓名:陈曦  赵建国
作者单位:江南大学工业生物技术教育部重点实验室,江苏,无锡,214036
摘    要:对采用米曲霉和酵母菌,利用混合酒糟生产含酶饲料蛋白的培养基组成和发酵条件进行了研究。结果表明:最优的培养基配比是啤酒糟:黄酒糟:豆粕:麸皮为4:1:1:4,初始料水比1:1.8,硫酸铵2%和磷酸盐1%,米曲霉在酵母菌培养16h时接入,总发酵周期68h。混株发酵与单株发酵相比,酵母数提高了3倍,平均粗蛋白提高5.32%,比基质粗蛋白高9.5%,粗蛋白增幅为35.74%,且发酵产物中含有蛋白酶901U/g(干曲),纤维素酶1824U/g(干曲)。

关 键 词:米曲霉  酵母  酒糟  粗蛋白
文章编号:0254-5071(2006)05-0030-04
修稿时间:2006年12月8日

Production of protein feed from a mixture of lees fermented by multi-strains
CHEN Xi,ZHAO Jian-guo.Production of protein feed from a mixture of lees fermented by multi-strains[J].China Brewing,2006(5):30-33.
Authors:CHEN Xi  ZHAO Jian-guo
Abstract:Using mixed strains of Aspergillus oryza and yeast,the influence of medium composition and fermentation condition on the production of feedstuff from a mixture of lees was investigated.It was found that the optimum medium composition was beers lees : rice wine lees: bean flour: wheat bran = 4:1:1:4,dry mass : water = 1:1.8,(NH4)2SO4 2%,KH2PO4 1%,inoculation time of Aspergillus oryza at 16 h,and fermentation time 68 h.Under this condition,in the medium with mixed strains,the yeast concentration was three times higher than that by single strain fermentation,and the crude protein(enhanced to 35.74%) was 9.5% higher than that of the original medium.Moreover,the feedstuff obtained from the fermentation with the mixed strains contained protease and cellulases with activity of 901 U/g and 1824 U/g(dry mass),respectively.
Keywords:Aspergillus oryza  yeast  lees  crude protein
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