| 干酪乳杆菌SB27降低N-亚硝胺对IEC-6细胞毒性作用研究 |
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| 引用本文： | 王淑梅,邸维,于佳睿,王雪,单艺,易华西.干酪乳杆菌SB27降低N-亚硝胺对IEC-6细胞毒性作用研究[J].中国酿造,2022,41(4):54-58. |
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| 作者姓名： | 王淑梅 邸维 于佳睿 王雪 单艺 易华西 |
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| 作者单位： | （1.哈尔滨学院 食品工程学院，黑龙江 哈尔滨 150086；2.广东科贸职业学院 食品与生物工程学院，广东 广州 510430； 3.中国海洋大学 食品科学与工程学院，山东 青岛 266100） |
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| 基金项目： | 黑龙江省博士后科研启动基金（LBH-Q21107）；;国家自然科学基金（31801516,32172180）；;广东省青年创新人才项目（2019GKQNCX042）； |
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| 摘 要： | 该研究选用具有益生功能的干酪乳杆菌（Lactobacillus casei）SB27为研究对象，采用菌落计数法分析N-亚硝胺（N-二甲基亚硝胺（NDMA）、N-二乙基亚硝胺（NDEA）、N-亚硝基吡咯烷（NPYR）和N-亚硝基哌啶（NPIP）（0～80 μg/mL）在MRS液体培养基中对干酪乳杆菌SB27生长的影响，并以维生素C（VC）（50 μmol/L）为阳性对照，采用细胞计数试剂盒（CCK）-8法分析干酪乳杆菌SB27（103～106 CFU/mL）降低N-亚硝胺对大鼠肠道黏膜细胞IEC-6毒性的损伤。结果表明，N-亚硝胺在MRS液体培养基中对干酪乳杆菌SB27的生长增殖无显著影响（P＞0.05）。干酪乳杆菌SB27可降低NDMA、NDEA、NPYR及NPIP对大鼠肠道黏膜细胞IEC-6的毒性损伤。
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| 关 键 词： | 干酪乳杆菌 N-亚硝胺 IEC-6细胞 毒性作用 |
Effect of Lactobacillus casei SB27 on reducing the cytotoxicity of N-nitrosamine to IEC-6 cells |
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| WANG Shumei,DI Wei,YU Jiarui,WANG Xue,SHAN Yi,YI Huaxi.Effect of Lactobacillus casei SB27 on reducing the cytotoxicity of N-nitrosamine to IEC-6 cells[J].China Brewing,2022,41(4):54-58. |
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| Authors: | WANG Shumei DI Wei YU Jiarui WANG Xue SHAN Yi YI Huaxi |
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| Affiliation: | (1.College of Food Engineering, Harbin University, Harbin 150086, China; 2.School of Food and Bioengineering, Guangdong Polytechnic of Science and Trade, Guangzhou 510430, China; 3.College of Food Science and Engineering, Ocean University of China, Qingdao 266100, China) |
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| Abstract: | Using Lactobacillus casei SB27 with probiotic function as research object, the effects of N-nitrosamine (N-dimethyl nitrosamine (NDMA), N-diethyl nitrosamine (NDEA), N-nitropyrrolidine (NPYR) and N-nitropiperidine (NPIP)) (0-80 μg/ml) on the growth of L. casei SB27 in MRS liquid medium were analyzed by colony counting method. Using vitamin (VC) (50 μmol/L) as positive control, the effect of L. casei SB27 (103-106 CFU/ml) on reducing the damage of intestinal mucosal IEC-6 cells induced by N-nitrosamine was analyzed by cell counting kit (CCK)-8 method. The results showed that N-nitrosamine had no significant effect on the growth and proliferation of L. casei SB27 in MRS liquid medium (P＞0.05). L. casei SB27 could reduce the toxic damage of NDMA, NDEA, NPYR and NPIP to IEC-6 cells. |
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| Keywords: | Lactobacillus casei N-nitrosamines IEC-6 cell toxic effect |
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