响应面法优化烟草发酵培养基提高细菌纤维素产量

为了提高细菌纤维素（BC）的产量，提升烟草废弃物的利用率，以木醋杆菌（Acetobacter xylinum）为试验菌株，静态发酵烟草废弃物制备细菌纤维素，通过单因素试验和Box-Behnken试验对烟草发酵培养基组分进行优化，并对细菌纤维素的持水性能进行分析。结果表明，烟草发酵培养基的最佳配方为：烟草废弃物浸提液1 L，硫酸铵含量3.2 g/L，乙醇体积分数2.0%，苹果酸含量0.5 g/L。在此优化条件下，BC产量为32.27 g/L，是优化前的2.74倍。优化后烟草发酵培养基生产的BC含水率为94.35%，与未优化培养基BC含水率（94.64%）相差不大，但其复水率和溶胀率分别为40.30%和673.56%，与未优化培养基相比，分别增加了8%和2%。

Optimization of tobacco fermentation medium by response surface methodology to improve bacterial cellulose yield

In order to increase the yield of bacterial cellulose (BC) and improve the utilization rate of tobacco waste, bacterial cellulose was prepared by static fermentation of tobacco waste using Acetobacter xylinum as experimental strain. The components of tobacco fermentation medium were optimized by single factor tests and Box-Behnken tests, and the water holding capacity of bacterial cellulose was analyzed. The results showed that the optimal formula of tobacco fermentation medium was as follows: tobacco waste extract 1 L, ammonium sulfate 3.2 g/L, ethanol volume fraction 2.0%, malic acid 0.5 g/L. Under the optimized conditions, the BC yield was 32.27 g/L, which was 2.74 times that of before optimization. The moisture content of BC produced by tobacco fermentation medium after optimization was 94.35%, which was not much different from the moisture content of BC (94.64%) produced by medium without optimization, but the rehydration rate and swelling rate were 40.30% and 673.56%, respectively, which increased 8% and 2% compared with medium without optimization.