西藏林芝地区不同品种葡萄果实表皮微生物群落结构组成分析

基于高通量测序技术对西藏林芝地区不同品种葡萄果实表皮细菌和真菌菌群的多样性及群落组成进行分析。结果表明，不同品种葡萄果实表皮微生物群落多样性差异明显，‘夏黑’葡萄果实表皮微生物丰富度和多样性指数最高，‘火焰无核’葡萄果实表皮最低。优势细菌属（相对丰度＞1%）主要有伯克霍尔德氏菌属（Burkholderia-Caballeronia-Paraburkholderia）、慢生根瘤菌属（Bradyrhizobium）、SJA-15、劳尔氏菌属（Ralstonia）、异样根瘤菌属（Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium）、寡养单胞菌属（Stenotrophomonas）、甲基杆菌属（Methylobacterium）和阿菲波菌属（Afipia），优势真菌属主要为球腔菌属（Mycosphaerella）、曲霉属（Aspergillus）、枝孢属（Cladosporium）和枝顶孢属（Acremonium）。结果显示，不同品种葡萄果实表皮微生物群落结构组成及相对丰度差异明显，说明葡萄品种影响葡萄果实表皮微生物群落结构组成。

Microbial community on the grape surface of different varieties from Nyingchi of Tibet

The bacterial and fungal diversity and community on the surface of different varieties of grape fruit grown in Nyingchi of Tibet were analyzed by high throughput sequencing technology. The results showed that there were significant differences in the microbial community of grape surface among different grape varieties, and the richness and diversity index of microbes on the grape surface of 'Summer Black' samples were the highest, while that were the lowest on the grape surface of Flame Seedless sample. The dominant bacteria genera (the relative abundance＞1%) were mainly Burkholderia-Caballeronia-Paraburkholderia, Bradyrhizobium, SJA-15, Ralstoni, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Stenotrophomonas, Methylobacterium and Afipia, and the dominant fungi genera mainly distributed in Mycosphaerella, Aspergillus, Cladosporium and Acremonium. The microbial community and relative abundance on the pericarp were different among different varieties of grape fruit, indicating that the grape varieties affected the microbial community on the surface of grape fruit.