葡聚糖酶产生菌的诱变选育

利用紫外线诱变育种方法分离对前期分离到一株葡聚糖酶野生菌株P5进行诱变。通过平板初筛、摇瓶复筛,从诱变菌株中筛选出一株高产菌株,其发酵酶活力可以达到5.85U/mL;在此基础上,对诱变菌株的发酵产酶工艺进行了初步的优化,其最适碳源为淀粉,最适氮源为花生饼粉,正交试验结果表明,淀粉含量为5%、花生饼粉含量为4%、NaH2PO4含量为0.2%时,该菌株的产酶量最高,酶活力达到21.23U/mL。

Mutation breeding of high glucanase producing strain

UV mutation breeding method was used to mutate a glucanase-producing wild strain P5 isolated in earlier stage. A high glucanase-producing strain was screened by plate screening and shaking flask culture with glucanase activity of 5.85 U/ml. Furthermore, the fermentation medium of mutant strain was optimized, and the optimum carbon source and nitrogen source was starch and peanut meal, respectively. The optimum medium formula was determined as follows： soluble starch 5%, peanut meal 4%, NaH2PO4 0.2%. Under these conditions, the fermentation glucanase activity reached up to 21.23 U/ml.