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搅拌转速和通气量对灰树花深层发酵培养的影响
引用本文:崔凤杰,陶文沂,许泓瑜,许正宏,敖宗华. 搅拌转速和通气量对灰树花深层发酵培养的影响[J]. 食品与生物技术学报, 2006, 25(4): 67-72
作者姓名:崔凤杰  陶文沂  许泓瑜  许正宏  敖宗华
作者单位:1. 江南大学,生物工程学院工业生物技术教育部重点实验室,江苏,无锡,214036;江南大学,生物工程学院生物制药研究室,江苏,无锡,214036
2. 江南大学,生物工程学院生物制药研究室,江苏,无锡,214036
基金项目:江苏省无锡市自然科学基金
摘    要:采用15L搅拌式发酵罐研究灰树花的扩大培养条件,主要考察了搅拌转速和通气量对灰树花菌丝量、胞外多糖产率、溶氧、菌丝形态以及发酵液粘度的影响.研究结果表明搅拌转速和通气量能显著影响灰树花的生长,并且通过对灰树花菌丝球的大小、形态以及胞外多糖的分泌影响发酵液的粘度.并得出较为优化的培养条件为:温度25℃,通气量0.75vvm,搅拌转速80r/min,装液量60%;在此条件下,发酵10d后,灰树花菌丝体最大得率为22.95g/L,胞外多糖的得率为1.521g/L。

关 键 词:灰树花  扩大培养  搅拌转速  通气量
文章编号:1673-1689(2006)04-0067-05
修稿时间:2005-09-17

Effects of Agitation and Aeration on the Production of Mycelial Biomass and Exo-polysaccharides by Grifola frondosa GF9801
CUI Feng-jie,TAO Wen-yi,XU Hong-yu,XU Zheng-hong,AO Zhong-hua. Effects of Agitation and Aeration on the Production of Mycelial Biomass and Exo-polysaccharides by Grifola frondosa GF9801[J]. Journal of Food Science and Biotechnology, 2006, 25(4): 67-72
Authors:CUI Feng-jie  TAO Wen-yi  XU Hong-yu  XU Zheng-hong  AO Zhong-hua
Abstract:The effects of cultivation agitation intensity and aeration rate on the production of mycelial biomass and exo-polysaccharides(EPS) by Grifola frondosa GF9801 in a 15 L batch fermenter were investigated in this study.Results revealed that agitation intensity and aeration rate could significantly influence the production of mycelial biomass and exo-polysaccharides(EPS),the concentrations of dissolved oxygen and reducing sugars,and the broth apparent viscosities.The highest mycelial biomass(22.95 g/L) and EPS concentration(1.521 g/L) were obtained at the optimized operating condition at agitation speed(80 r/min) and aeration rate(0.75 vvm) after 10-day cultivation.
Keywords:Grifola frondosa  batch fermentation  agitation intensity  aeration rate
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