| 搅拌转速和通气量对灰树花深层发酵培养的影响 |
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| 引用本文： | 崔凤杰,陶文沂,许泓瑜,许正宏,敖宗华. 搅拌转速和通气量对灰树花深层发酵培养的影响[J]. 食品与生物技术学报, 2006, 25(4): 67-72 |
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| 作者姓名： | 崔凤杰 陶文沂 许泓瑜 许正宏 敖宗华 |
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| 作者单位： | 1. 江南大学,生物工程学院工业生物技术教育部重点实验室,江苏,无锡,214036;江南大学,生物工程学院生物制药研究室,江苏,无锡,214036
2. 江南大学,生物工程学院生物制药研究室,江苏,无锡,214036 |
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| 基金项目： | 江苏省无锡市自然科学基金 |
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| 摘 要： | 采用15L搅拌式发酵罐研究灰树花的扩大培养条件，主要考察了搅拌转速和通气量对灰树花菌丝量、胞外多糖产率、溶氧、菌丝形态以及发酵液粘度的影响．研究结果表明搅拌转速和通气量能显著影响灰树花的生长，并且通过对灰树花菌丝球的大小、形态以及胞外多糖的分泌影响发酵液的粘度．并得出较为优化的培养条件为：温度25℃，通气量0．75vvm，搅拌转速80r／min，装液量60％；在此条件下，发酵10d后，灰树花菌丝体最大得率为22．95g／L，胞外多糖的得率为1．521g／L。
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| 关 键 词： | 灰树花 扩大培养 搅拌转速 通气量 |
| 文章编号： | 1673-1689(2006)04-0067-05 |
| 修稿时间： | 2005-09-17 |
Effects of Agitation and Aeration on the Production of Mycelial Biomass and Exo-polysaccharides by Grifola frondosa GF9801 |
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| CUI Feng-jie,TAO Wen-yi,XU Hong-yu,XU Zheng-hong,AO Zhong-hua. Effects of Agitation and Aeration on the Production of Mycelial Biomass and Exo-polysaccharides by Grifola frondosa GF9801[J]. Journal of Food Science and Biotechnology, 2006, 25(4): 67-72 |
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| Authors: | CUI Feng-jie TAO Wen-yi XU Hong-yu XU Zheng-hong AO Zhong-hua |
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| Abstract: | The effects of cultivation agitation intensity and aeration rate on the production of mycelial biomass and exo-polysaccharides(EPS) by Grifola frondosa GF9801 in a 15 L batch fermenter were investigated in this study.Results revealed that agitation intensity and aeration rate could significantly influence the production of mycelial biomass and exo-polysaccharides(EPS),the concentrations of dissolved oxygen and reducing sugars,and the broth apparent viscosities.The highest mycelial biomass(22.95 g/L) and EPS concentration(1.521 g/L) were obtained at the optimized operating condition at agitation speed(80 r/min) and aeration rate(0.75 vvm) after 10-day cultivation. |
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| Keywords: | Grifola frondosa batch fermentation agitation intensity aeration rate |
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