刺梨果汁对拘束负荷诱发小鼠肝损伤的保护作用

研究新鲜刺梨果汁和刺梨胶原饮料对拘束负荷诱发小鼠肝损伤的保护作用。将雄性昆明种小鼠分为正常对照组、应激模型组、刺梨果汁组,刺梨胶原饮料组,每天灌胃给药一次,连续7 d,最后一次给药30 min后模型组和给药组给予一次性拘束负荷18 h。检测小鼠血浆谷丙转氨酶(ALT)水平、抗氧化能力指数(ORAC)、肝组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)含量以及刺梨果汁和胶原饮料的体外抗氧化水平。结果与应激模型组相比,刺梨果汁及胶原饮料明显降低小鼠血浆ALT水平和肝组织中MDA含量,改善血浆ORAC及肝组织SOD活性。其中,刺梨胶原饮料还能显著提高肝组织GSH含量。此外,体外实验结果证明刺梨果汁及胶原饮料均显示一定的抗氧化活性。刺梨果汁和刺梨胶原饮料对拘束负荷诱发的小鼠肝损伤具有一定的保护作用,其作用机制可能部分来自于清除自由基和抑制脂质过氧化过程。

Protective Effect of Rosa roxburghii Tratt Drink Against Liver Injury in Mice Loaded with Restraint Stress

OBJECTIVE To investigate the protective effect of Rosa roxburghii Tratt juice(100% raw juice)and Rosa roxburghii Tratt collagen drink on restraint-stress induced liver injury of mice.METHODS The male KM mice were randomly divided into normal control group,stress model group,Rosa roxburghii Tratt juice group and Rosa roxburghii Tratt collagen drink group.The mice were orally administered samples once a day successively for 7 days,then restrained for 18 h after 30 min of the last intragastric administration.Datum were obtained including activities of Alanine aminotransferase(ALT) and the Oxygen radical absorbance capacity(ORAC)value in plasma,the content of Malondialdehyde(MDA),glutathione(GSH) and activities of superoxide dismutase(SOD) in liver.Antioxidant capacity of Rosa roxburghii Tratt juice and collagen drink in vitro were also determined.RESULTS Rosa roxburghii Tratt juice and collagen drink can markedly decrease activities of ALT in plasma,reduced MDA level in liver and effectively increase ORAC value in plasma of mice compared with the model group.They can also increase SOD activities in mice liver.Apart from the above roles,Rosa roxburghii Tratt collagen drink can increase GSH content in mice liver compared with model group.Additionally,Rosa roxburghii Tratt juice and collagen drink can effectively increase the ORAC value in vitro.CONCLUSION The results indicate that Rosa roxburghii Tratt and collagen drink had the effect of protecting liver from damage induced by stress in mice.It is considered that this protective effect may be related to its free radical scavenging activity and lipid peroxidation inhibitory effects.