无溶剂体系合成1,3-甘油二酯用脂肪酶的筛选及其酯化性质

对不同来源的脂肪酶进行了系统的筛选,并根据位置专一性的差别将脂肪酶分为1,3 特异的、1,3 选择性和非选择性的3类.采用LipozymeRMIM在无溶剂系统中催化甘油、脂肪酸合成1,3 sn 甘油二酯.研究表明脂肪酸、底物醇种类、温度、水分活度对LipozymeRMIM的活力有明显的影响.

Screening of Lipase for Synthesis of 1,3-sn-Diacylglycerols and Its Esterification Characteristics in Solvent-Free System

Lipases from different commercial sources were screened in terms of their regioselectivities (RE) and activity of esterification. Obvious differences were found in the RE value so that it can be selected as a measure to classify these biocatalysts as those of specific, selective, and nonspecific. On the base of systematic screening experiments, Lipozyme RM~(IM) indicating high regioselectivites and high esterification activity was used as the catalyst for efficient synthesis of 1,3-sn-Diglyceride with glycerol and fatty acid as the raw materials in solvent-free system . The results suggested that the species of fatty acids and alcohols, reaction temperature, and water activity effected esterification activity of Lipozyme RM~(IM) significantly.