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萌发大麦种子内肽酶活力、醇溶蛋白及氨基酸含量变化
引用本文:邱然,杨文洲,张春玲,赵长新,闵建.萌发大麦种子内肽酶活力、醇溶蛋白及氨基酸含量变化[J].食品与生物技术学报,2007,26(5):48-52.
作者姓名:邱然  杨文洲  张春玲  赵长新  闵建
作者单位:1. 大连工业大学,生物与食品工程学院,辽宁,大连,116034
2. 东北电力大学应用技术学院,吉林省吉林,130012
3. 大连产品质量监督检验所,辽宁,大连,116021
基金项目:辽宁省高等学校重点实验室基金
摘    要:以两个大麦品种垦啤8号和Harrington为材料研究了大麦种子萌发过程中体内醇溶性蛋白质、游离氨基酸和内肽酶活力的变化。垦啤8号大麦种子的醇溶蛋白含量高,但其降解量比Harrington多。垦啤8号游离氨基酸增长量略高于Harrington。整个大麦种子发芽期间,国产品种内肽酶活力始终高于进口品种。以上表明,与Harrington相比,虽然国产品种垦啤8号含氮量高,但其内肽酶活力升高快,最大酶活力高,醇溶蛋白降解、转化为可输出氮的进程快,有制造优质麦芽的潜力。

关 键 词:萌发  内肽酶  醇溶蛋白  游离氨基酸
文章编号:1673-1689(2007)05-0048-05
收稿时间:2006-11-29
修稿时间:2006年11月29

Changes in Endopeptidase Activity, Content of Hordein and Free Amino Acids in Barley Seeds during Germination
QIU Ran,YANG Wen-zhou,ZHANG Chun-ling,ZHAO Chang-xin,MIN Jian.Changes in Endopeptidase Activity, Content of Hordein and Free Amino Acids in Barley Seeds during Germination[J].Journal of Food Science and Biotechnology,2007,26(5):48-52.
Authors:QIU Ran  YANG Wen-zhou  ZHANG Chun-ling  ZHAO Chang-xin  MIN Jian
Affiliation:1. College of Biotechnology and Food Technology , Dalian Polytechnic university, Dalian 116034, China; 2. Northeast DianLi University, Jilin 130012, China; 3. Dalian Product Quality Supervision and Testing Institute, Dalian 116021, China
Abstract:Two species barleys, Hordeum sp. Kenpi No. 8 and Hordeum sp. Harringto were used to study changes in contents of hordein, free amino acids and endopeptidase activity during germination. Results showed that the content of hordein in Kenpi 8 was higher than that of Harrington, but decreased faster. The same tendency of free amino acids and endopeptidase activity appeared in the two species barleys during germination. It implied that, compared with Harrington, the procedures of hordein break down and transfer to transportable nitrogen in Kenpi 8 was higher and faster during germination, and this maybe an important reason of Kenpi 8 has potential to make high-quailty malt.
Keywords:germination  endopeptidase  hordein  free amino acid
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