高产类胡萝卜素红酵母的筛选及其发酵条件

通过对数株红酵母培养后发酵液中菌体生物量及类胡萝卜素含量的测定比较，从中选出了1株产类胡萝卜素能力较强的红酵母RY-98；研究了该菌株产类胡萝卜素的最适碳源和氮源，并对其主要营养与环境条件进行了选择及优化，获得了最佳发酵条件葡萄糖40g/L、(NH

Screening of Rhodotorula Producing Carotenoidsand Its Fermentation Conditions

The Rhodotorula strain RY-98 with high carotenoids yield of 6.7 mg/L was obtained from 12 strains of yeasts by common screening test. The varied physiological conditions for carotenoids fermentation were studied by uniform design test method. The cell biomass, carotenoids content and yield from strain RY-98 could respectively reach 26.8 g/L, 386.9 μg/g and 10.4 mg/L under selected optimum conditions. The optimum fermentation conditions for carotenoids production were as follows, glucose 40 g/L,(NH4）2SO4　10 g/L, yeast extract 3 g/L, tomato juice 2 mL/L, peanut oil 0.5 mL/L, medium 40 mL(in 250 mL flask), initial pH 6.0, inoculum size 30 mL/L and culture time 72 h(at 28 ℃， 150 r/min).