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血红蛋白降解菌的分离筛选与鉴定
引用本文:陶艳华,姚大伟,王政,郭士兵,李臻兴,杨德吉.血红蛋白降解菌的分离筛选与鉴定[J].食品与生物技术学报,2009,28(6):854-857.
作者姓名:陶艳华  姚大伟  王政  郭士兵  李臻兴  杨德吉
作者单位:1. 南京农业大学,动物医学院,江苏,南京,210095
2. 江西省种鸡场,江西,南昌,330220
摘    要:为了从环境中筛选高效降解血红蛋白的菌株,采用鲜血琼脂平板,以产生溶血圈比值大小为判定标准,从南京某屠宰场下水道中分离到一株具有较强降解血红蛋白能力的菌株NJM4.经形态学观察、生理生化测定和16S rRNA基因分析(GenBank登陆号为EU234500),初步认为该菌株为短小芽孢杆菌(Bacillus pumilus).该菌株在血红蛋白溶液中可将其逐渐降解为小分子的多肽.在血红蛋白发酵培养基中于37℃、120 r/min培养24 h,降解率达53.64 %.

关 键 词:血红蛋白  降解  分离  鉴定

Isolation and Identification of Hemoglobin Degradation Microorganism
TAO Yan-hu,YAO Da-wei,WANG Zheng,GUO Shi-bing,LI Zhen-xing and YANG De-ji.Isolation and Identification of Hemoglobin Degradation Microorganism[J].Journal of Food Science and Biotechnology,2009,28(6):854-857.
Authors:TAO Yan-hu  YAO Da-wei  WANG Zheng  GUO Shi-bing  LI Zhen-xing and YANG De-ji
Abstract:To obtain high efficient degradation bacteria of hemoglobin, strain NJM4 from slaughterhouse sewer in Nanjing was isolated by blood agar plate. The ability of degeneration hemoglobin was assessed by hematolysis circle ratio. Initially, the strain was identified as Bacillus pumiius based on morphology observation, physicochemical characteristics and 16S rRNA sequence analysis (GenBank ID: EU234500). The results showed that the hemoglobin could be degraded into small molecular multi-peptides, and after 24 h incubation under 37 ℃ , 120 r/min, the degradation rate of hemoglobin could reach to 53. 64%.
Keywords:hemoglobin  degradation  separation  identification
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