| 三聚氰胺单克隆抗体的制备及其免疫学特性鉴定 |
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| 引用本文： | 钮伟民,何恩奇,戴维杰,赵春城,刘文卫,杨婷婷.三聚氰胺单克隆抗体的制备及其免疫学特性鉴定[J].食品与生物技术学报,2011,30(6):884-886. |
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| 作者姓名： | 钮伟民 何恩奇 戴维杰 赵春城 刘文卫 杨婷婷 |
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| 作者单位： | 1. 无锡市疾病预防控制中心,江苏无锡,214023
2. 江苏省苏微微生物研究所,江苏无锡,214036 |
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| 基金项目： | 无锡市卫生系统科研基金项目(XM0906) |
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| 摘 要： | 采用牛血清白蛋白偶联的三聚氰胺( melamine- BSA)完全抗原免疫BALB/C小鼠,应用杂交瘤技术制备抗三聚氰胺的特异性单克隆抗体.所得单克隆抗体效价可达5.0×105,且抗体的亚类类型为IgG1,抗体的亲和常数为3.21×109 L/mol.与三聚氰酸、三聚氰酸二酰胺、三聚氰酸一酰胺的交叉反应率分别为30％、93％和46％.说明该单抗可用于三聚氰胺类物质的检测,为研制高质量的三聚氰胺ELISA试剂盒和胶体金试纸条奠定了基础.
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| 关 键 词： | 三聚氰胺 单克隆抗体 鉴定 免疫学检测 |
Preparation and Immunological Characterization of Monoclonal Antibody Against Melamine |
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| NIU Wei-min,HE En-qi,DAI Wei-jie,ZHAO Chun-cheng,LIU Wen-wei and YANG Ting-ting.Preparation and Immunological Characterization of Monoclonal Antibody Against Melamine[J].Journal of Food Science and Biotechnology,2011,30(6):884-886. |
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| Authors: | NIU Wei-min HE En-qi DAI Wei-jie ZHAO Chun-cheng LIU Wen-wei and YANG Ting-ting |
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| Affiliation: | NIU Wei-min1,HE En-qi1,DAI Wei-jie1,ZHAO Chun-cheng2,LIU Wen-wei1,YANG Ting-ting2(1.Wuxi Center for Disease Control and Prevention,Wuxi 214023,China,2 Jiangsu suwei Institute of Microbiology CO.LTD,Wuxi 214036,China) |
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| Abstract: | In this study,mice BALB/C were immunized by melamine-BSA and anti-melamine monoclonal antibody(McAb) was prepared by using hybridoma technique.The ELISA titers of ascite was exceeded 50,0000.Subtype of the McAb was IgG1 and the affinity constant was 3.21 109 L/mol.The cross-reactivity with Cyanuric acid,cyanurodiamide and ammelide was 30%,93% and 46% respectively.The monoclonal antibody can be used for fast determination of melamine substances. |
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| Keywords: | melamine monoclonal antibody characterization immunological detection |
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