| 比色法测定胆固醇氧化酶酶活 |
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| 引用本文： | 季文明,陈毅力,张和春,王武.比色法测定胆固醇氧化酶酶活[J].食品与生物技术学报,2000,19(3):251-254. |
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| 作者姓名： | 季文明 陈毅力 张和春 王武 |
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| 作者单位： | 无锡轻工大学生物工程学院,江苏无锡,214036 |
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| 基金项目： | 国家轻工局轻工科技发展计划项目! (G0 3- 1 3) |
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| 摘 要： | 根据实验结果和理论计算确定检测体系中胆固醇的质量浓度为 0 .3 87mg/mL ,表面活性剂的质量浓度为 0 .2 g/dL ,确定过氧化氢浓度与反应液吸光度之间的定量关系 .在此基础上 ,建立了比色法测定胆固醇氧化酶酶活的方法 .
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| 关 键 词： | 胆固醇氧化酶 胆固醇 比色法检测 |
Colorimetric Determination of the Activity of Cholesterol Oxidase |
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| JI Wen-ming,CHEN Yi-li,ZHANG He-chun,WANG Wu.Colorimetric Determination of the Activity of Cholesterol Oxidase[J].Journal of Food Science and Biotechnology,2000,19(3):251-254. |
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| Authors: | JI Wen-ming CHEN Yi-li ZHANG He-chun WANG Wu |
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| Abstract: | In the paper,Determined the choleterol and the detergent in the final solution were determined as 0.387 mg/mL and 0.2 g/dL respectively. A linear equation about the quantitative relationship between the hydrogen peroxide and the absorbency was set up.The colorimetric determination method of the activity of cholesterol oxidase was established. |
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| Keywords: | cholesterol oxidase cholesterol colorimetric determination |
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