OCC稀酸水解液发酵制取乙醇的研究

在硫酸质量分数4%、温度190℃、时间40min、液固比15（ml/g）、助催化剂（FeSO4）1%情况下以二次纤维（OCC）为原料制取水解液。在温度28℃、转速150r/min、接种量10%条件下,选取酿酒酵母（CICC1001）对水解液发酵制取乙醇进行了研究,考察了菌种驯化、水解液过中和处理以及助催化剂（FeSO4）对乙醇产量的影响。结果表明：驯化酿酒酵母可以提高OCC水解液发酵速率以及乙醇产量;以Ca（OH）2过中和处理后产乙醇量明显优于中和处理,最高乙醇产量比中和处理可高出85.20%;加入FeSO4的水解液与未加入FeSO4水解液发酵规律一致,而未加入FeSO4水解液发酵产乙醇量滞后于加入FeSO4的水解液。

Studies on ethanol prepared from OCC hydro-lyzate produced by dilute acid hydrolysis

Hydrolyzate of old corrugated carton was chosen as fermentation materials which were obtained by dilute acid hydrolysis under the condition of mass fraction of sulfuric acid 4%,reaction temperature 190℃,reaction time 40min,liquid-solid ratio 15ml/g,catalyst FeSO4 1%.Ethanol production was studied by using Saccharomyces cerevisiae（CICC1001） under the condition of temperature 28℃,rotating speed 150r/min,inoculation rate 10%.Simultaneously the influences on ethanol yield such as yeast-training,Ca（OH）2 over-neutralization,catalyst（FeSO4） were carried out.The results show that yeasttraining can improve the speed of the OCC hydrolyzate fermentation and ethanol yield;the over-neutralization of the OCC hydrolyzate by Ca（OH）2 is superior to the direct neutralization treatment by Ca（OH）2 in ethanol yield and can produce 85.20% more ethanol than the direct neutralization treatment.The OCC hydrolyzate of using FeSO4 is in accordance with not using FeSO4 in the respect of producing ethanol,but the OCC hydrolyzate of not using FeSO4 lagged behind the OCC hydrolyzate of using FeSO4.