| 利用蛋白质"指纹"技术鉴定加拿大啤酒大麦的品种和纯度 |
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| 引用本文： | 林艳,梅承芳,董建军,周志娟.利用蛋白质"指纹"技术鉴定加拿大啤酒大麦的品种和纯度[J].啤酒科技,2006,199(2):12-15. |
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| 作者姓名： | 林艳 梅承芳 董建军 周志娟 |
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| 作者单位： | 1. 中国海洋大学生命科学与技术学部,266003;青岛啤酒股份有限公司科研中心,266061
2. 青岛啤酒股份有限公司科研中心,266061 |
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| 摘 要： | 开发了操作简便、电泳图谱清晰、分辨率高的啤酒大麦种子醇溶蛋白SDS—PAGE电泳方法，利用Gel—Pro软件对电泳图谱进行条带分析和比较，建立了每个品种的蛋白质“指纹”，组成加拿大啤酒大麦品种标准图谱库。利用Cross Checker2．8和MEGA3软件对供试啤麦品种进行遗传聚类分析，结果与传统分类相近。进行了人工混种试验，并成功将该技术应用于公司2004年进口加拿大啤麦的品种和纯度鉴定。
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| 关 键 词： | 蛋白质"指纹"技术 啤酒大麦 SDS-PAGE电泳 品种和纯度鉴定 |
| 收稿时间： | 2005-10-12 |
| 修稿时间： | 2005年10月12 |
Variety and Purity Identification of Malting Barley by Protein Fingerprint Technique |
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| Lin Yan,Mei Chengfang,Dong jianjun,Zhou Zhijuan.Variety and Purity Identification of Malting Barley by Protein Fingerprint Technique[J].Beer Science and Technology,2006,199(2):12-15. |
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| Authors: | Lin Yan Mei Chengfang Dong jianjun Zhou Zhijuan |
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| Abstract: | A method of SDS-PAGE electrophoresis was developed to identify genuineness of seed and cuhivar purity of malting barley. By analyzing the electrophorograms by the Gel-Pro software, protein fingerprint of each cuhivar was obtained, which composed the standard fingerprint bank of Canadian malting barley. The dendrogram of the 8 tested cultivars by the application of Cross Checker 2.8 and MEGA 3 softwares was consistent with the traditional classification result. Test of mixing cultivars artificially was done, the result indicated that this technique could be applied to identify variety and purity of imported malting barley. |
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| Keywords: | protein fingerprint technique malting barley SDS-PAGE electrophoresis variety and purity identification |
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