Detection of clenbuterol in beef meat,liver and kidney by mid‐infrared spectroscopy (FT‐Mid IR) and multivariate analysis |
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Authors: | Ofelia G Meza‐Márquez Tzayhrí Gallardo‐Velázquez Guillermo Osorio‐Revilla Lidia Dorantes‐Álvarez |
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Affiliation: | 1. Departamento de Graduados e Investigación en Alimentos, Escuela Nacional de Ciencias Biológicas, IPN, , Santo Tomás, CP, 11340 México, DF;2. Departamento de Biofísica, Escuela Nacional de Ciencias Biológicas, IPN, , Santo Tomás, CP, 11340 México, DF;3. Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, IPN, , Santo Tomás, CP, 11340 México, DF |
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Abstract: | Mid‐infrared spectroscopy (FT‐Mid IR) coupled with multivariate analysis was used to predict clenbuterol in beef meat, liver and kidney. A SIMCA model was also developed to discriminate between pure (beef meat, liver and kidney) and spiked with clenbuterol samples (beef meat‐clenbuterol, liver‐clenbuterol and kidney‐clenbuterol). The best models to predict clenbuterol concentrations were obtained using the partial least squares algorithm (PLS) with a R2 > 0.9 and SEC and standard error of prediction <0.296 and 0.324, respectively. The SIMCA model used to discriminate pure and spiked with clenbuterol samples showed 100% correct classification rate. Methods detection limit was 2 μg kg?1. FT‐Mid IR coupled with chemometrics could be a simple and rapid screening tool for monitoring clenbuterol in beef meat, liver and kidney implicated in food poisoning. This method could be use for screening purposes. |
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Keywords: | Chemometrics clenbuterol FT‐Mid IR
SIMCA
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