Effects of aminoguanidine on γ-aminobutyric acid accumulation pathways in fresh tea leaves under hypoxic conditions |
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| Authors: | Qinyan Wu Rui Guo Wenwen Zhang Dongdong Li Bing Rao Shengzhou Ma Fei Zhao Kebing Yao Yiqing Zhuang |
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| Affiliation: | 1. Zhenjiang Institute of Agricultural Sciences in Hill Area of Jiangsu Province, Jurong, Jiangsu, 212400 China;2. Agricultural Committee of Zhenjiang City, Jurong, Jiangsu, 212400 China;3. Jiangsu Vocational College of Agriculture and Forestry, Jurong, Jiangsu, 212400 China |
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| Abstract: | Aminoguanidine (AG), a specific inhibitor of diamine oxidase (DAO), can be used to investigate the γ-aminobutyric acid (GABA) accumulation pathways. The effect of AG on GABA accumulation pathways of tea fresh leaves remains to be explored. Our results indicated AG concentration did not significantly affect GAD activity, but high concentration solution made GABA-T activity increase. AG (1 mm ) maximally, but not entirely, blocked the polyamine degradation pathway, but had no effect on GAD and GABA-T. The polyamine degradation pathway was responsible for 26.8% and 17.0% of GABA formation under hypoxic conditions involving immersion and immersion–vacuum, respectively, because DAO and PAO activity was not completely inhibited. |
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| Keywords: | Aminoguanidine fresh tea leaves GABA shunt polyamine degradation γ-aminobutyric acid |
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