Enhanced β-galactosidase production by supplementing whey with cauliflower waste |
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Authors: | Harinder Singh Oberoi Sunil Bansal & Gurpreet Singh Dhillon |
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Affiliation: | Central Institute of Post Harvest Engineering and Technology, P.O. PAU, Ludhiana 141 004, India |
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Abstract: | Among the five Kluyveromyces marxianus strains tested for β-galactosidase production, K. marxianus NCIM 3465 showed maximum enzyme activity of 1.62 IU mg?1 dry weight. Different levels (5–25%, w/v) of dried cauliflower waste were incorporated into whey to evaluate the effect of its supplementation on enzyme production. Although a marginal increase in enzyme production was seen by incorporating 5% and 10% cauliflower waste in whey, nearly 15% increase in β-galactosidase production was observed when cauliflower waste level was increased to 20% compared with whey alone. Supplementing whey with 20% cauliflower waste also decreased the production time. Lactose concentration in whey, mainly responsible for increasing the biological oxygen demand load of the effluent water, decreased from 4.2% to nearly 0% at 24 h. Thus, this study demonstrated that both these by-products/residues could be effectively used for β-galactosidase production at commercial scale. |
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Keywords: | Biomass cauliflower waste β-galactosidase activity lactose concentration whey |
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