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Fresh beef tumbling at different post-mortem times to improve tenderness and proteolytic features of M.?longissimus lumborum
Authors:Mariah J Nondorf  Yuan H Brad Kim
Affiliation:1. Meat Science and Muscle Biology Laboratory, Department of Animal Sciences, Purdue University, West Lafayette, IN, 47907 USA

Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Methodology (equal), Visualization (equal), Writing - original draft (equal);2. Meat Science and Muscle Biology Laboratory, Department of Animal Sciences, Purdue University, West Lafayette, IN, 47907 USA

Abstract:The aim of this study was to determine the effects of tumbling at different post-mortem times on the proteolytic features and quality attributes of beef loins (M. longissimus lumborum). Loins (n = 12) were cut into 4 sections and assigned to tumbling at 1, 6 or 11 days post-mortem or non-tumbled control. Upon tumbling, additional ageing was applied to a common post-mortem time of 16 days. In general, tumbling had no considerable impacts on water-holding ability of samples. Tumbling resulted in an immediate decrease in shear force values (WBSF) of beef samples. Tumbling at 1 day post-mortem with no ageing had similar WBSF compared to the non-tumbled controls at 16 days (P > 0.05). With ageing, tumbling increased amounts of protein degradation, myofibrillar fragmentation and calpain-1 autolysis of samples. These results suggest that early post-mortem tumbling coupled with ageing can synergistically impact the tenderness development of beef loins and shorten the necessary ageing period.
Keywords:Beef quality  calpain activation  post-mortem proteolysis  tenderness  tumbling
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