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Adaptive response and tolerance to weak acids in Saccharomyces cerevisiae boulardii: a metabolomics approach |
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| Authors: | Junjie Fu Chaolan Liu Li Li Jun Liu Yu Tie Xueping Wen Qikai Zhao Zongwei Qiao Zheming An Jia Zheng |
| Affiliation: | 1. College of Biotechnology Engineering, Sichuan University of Science and Engineering, Yibin, 644000 China;2. Antibiotics Research and Re-evalution Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, 610052 China
Contribution: Software (equal), Validation (equal), Writing - original draft (equal);3. College of Biotechnology Engineering, Sichuan University of Science and Engineering, Yibin, 644000 China Solid-State Fermentation Resource Utilisation Key Laboratory of Sichuan Province, Yibin, 644000 China Contribution: Methodology (equal), Validation (equal), Visualization (equal);4. College of Biotechnology Engineering, Sichuan University of Science and Engineering, Yibin, 644000 China Contribution: Data curation (equal), ?Investigation (equal), Methodology (equal), Software (equal);5. College of Biotechnology Engineering, Sichuan University of Science and Engineering, Yibin, 644000 China HengfengHuaBang Biological Science and Technology Co., Ltd., Leshan, 614000 China Contribution: Data curation (equal);6. Wuliangye Yibin Co, Ltd, Yibin, 644000 China |
| Abstract: | Weak acids inhibit the growth of probiotics, such as Saccharomyces boulardii. We explored the tolerance of S. boulardii to different weak acids. S. boulardii had better fermentation ability under lactic acid conditions compared with acetic and butyric acid conditions; however, the budding of S. boulardii was significantly stronger than that of Saccharomyces cerevisiae under acetic acid conditions. Although the surface structure of S. boulardii was destroyed, it produced more daughter cells. S. boulardii metabolites were also significantly different from S. cerevisiae under acidic stress. The growth of S. boulardii under weak acid conditions differed significantly from that of S. cerevisiae. S. boulardii-mediated fingerprints under weak acid conditions were identified as latent biomarkers, related to fructose and mannose metabolism, tricarboxylic acid cycle, and the glycolysis pathway. Identified biomarkers will aid in the genetic engineering of S. boulardii and other Saccharomyces strains for improved acid resistance and biomass yield. |
| Keywords: | Growth metabolomics S boulardii S cerevisiae weak acid stress |