甘蓝型油菜(Brassica napus L.)RIL群体抗倒伏及其相关性状的QTL分析 |
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引用本文: | 李扬,顾慧,戚存扣. 甘蓝型油菜(Brassica napus L.)RIL群体抗倒伏及其相关性状的QTL分析[J]. 中国油料, 2014, 0(1): 10-17 |
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作者姓名: | 李扬 顾慧 戚存扣 |
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作者单位: | [1]南京农业大学农学院,江苏南京210095 [2]江苏省农业科学院经济作物研究所,江苏南京210014 |
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基金项目: | 农业部948重大项目(2011-G23);国家科技支撑计划(2010BAD01810);国家油菜产业技术体系(CARS-13);江苏省农业科技自主创新资金[CX(11)4009] |
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摘 要: | 油菜倒伏严重影响了油菜的产量和品质,为了定位甘蓝型油菜(Brassica napus L.)与抗倒伏性状相关的QTL,用抗倒伏品系浙平1号和易倒伏品系高芥1号组合重组自交系(RIL)家系为材料,通过两年两点试验,对该RIL群体的F2:6和F2:7,家系抗压力性状进行了QTL分析,并应用一年两点数据对该群体F2:6。家系结角层性状进行了QTL分析。结果表明,在4个试验中共检测到11个家系抗压力QTL,可解释4.41%~52.64%的表型变异,LOD值范围是2.68~26.58。其中qRP16-1可以在3个不同的试验中(南京-2012、溧水-2012和南京-2013)重复检测到,面P1、qRP4和qRP8-1同时在2个不同的试验中(溧水-2012和溧水-2013)重复检测到。qRP2-1、qRP2-2、qRP8-2、qRP8—3、qRPl6—2、qRP3和qRP8—4等7个QrL只能在特定的试验中检测到。同一地点不同年份间家系抗压力性状QTL相对同一年份不同地点间易被重复检测到。在2个试验中共检测到2个结角层厚度QTL,位于第15连锁群的qTPLl5在2个试验中重复检测到。
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关 键 词: | 甘蓝型油菜 重组近交系 抗倒伏 抗压力 结角层 QTLs |
QTL mapping for lodging resistance and its related traits by RIL population of Brassica napus L. |
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Affiliation: | LI Yang, GU Hui, QI Cun- kou( 1. Agronomy College of Nanjing Agricultural University, Nanjing 210095, China ; 2. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 21 O014, China) |
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Abstract: | Lodging causes yield loss and quality deficiency severely in oilseed rape. To mapping QTLs related to lodging resistance, 243 lines of a recombinant inbred line (RIL) population ( F2:6 and F2:7 ) of Brassica napus L. were tested at two locations in two consecutive years. The RIL population was derived from Zheping 1 (lodging resistant) x Gaojie 1 (lodging susceptible). Results showed 11 QTLs for plant pressure resistance (PPR) using inclusive composite interval mapping from 4 trials. Their phenotype variation and LOD value ranges were 4.41% - 52.64% and 2.68 -26.58 respectively. Among QTLs, qRPI6 -1 was co -localized by 3 trials (Nanjing- 2012, Lishui - 2012 and Lishui - 2013 ). qRP1, qRP4 and qRP8 - 1 were co - localized by 2 trials ( Lishui - 2012 and Lishui-2013). And qRP2- 1, qRP2-2, qRP8-2, qRP8-3, qRP16-2, qRP3 and qRP8-4 were only local- ized by one trial. QTLs for PPR were co -localized more easily at single location in each year than those in single year at each location. Two QTLs for thickness of pod - set layer were identified from 2 trials, qTPL15 which on LG15 was eo -localized by 2 trials. |
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Keywords: | Brassica napus L. Recombinant inbred line (RIL) Lodging resistance Pressure resistance Pod - set layer QTLs |
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