Changes of phosphorus and trace elements species in rye and oat flakes and oat porridge induced by simulated digestion |
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Authors: | Richard Koplík Martina Linková Oto Mestek |
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Affiliation: | 1.Institute of Chemical Technology,Prague 6,The Czech Republic |
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Abstract: | Fractionation of chemical species of phosphorus, manganese, iron, nickel, copper, zinc and molybdenum in rye and oat flakes
and porridge made of oat flakes was studied by size exclusion chromatography coupled to inductively coupled plasma mass spectrometry.
The effect of in vitro simulated digestion of the samples was elucidated by comparison of the digestates and the extracts
of the samples (in the Tris–HCl buffer solution, pH = 7.5). The procedure of simulated digestion included two steps: the pepsin-catalysed
hydrolysis in diluted hydrochloric acid (pH = 1.7) followed by the pancreatin-catalysed hydrolysis at pH = 7.5. The enzyme-catalysed
digestion of the samples results in alteration of iron, copper and zinc species distribution. The species of phosphorus in
original cereal samples are distributed between two fractions: a medium-molecular mass fraction (15 kDa) and a low-molecular
mass fraction (2 kDa). This pattern is also kept basically in the sample digestates. In digested samples, the majority of
soluble iron is bound in a medium-molecular mass fraction (20 kDa), while in the extracts only traces of iron were found in
a high-molecular mass fraction. Approximately 90% of the soluble zinc in the digestates of cereal flakes is represented by
non-bound zinc ions. The bound zinc is mostly concentrated in a low-molecular mass fraction (1–2 kDa) together with some copper
and almost all nickel. The low-molecular mass fractions (1–2 kDa) of nickel, copper and zinc present in extracts of original
samples correspond quite well to those in the respective digestates. Molybdenum species occurs in another low-molecular mass
fraction. |
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