| 酶法甘油解制备甘油二酯的研究 |
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| 引用本文： | 王卫飞,宁正祥,徐扬,王永华,杨博.酶法甘油解制备甘油二酯的研究[J].中国油脂,2012,37(5):31-34. |
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| 作者姓名： | 王卫飞 宁正祥 徐扬 王永华 杨博 |
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| 作者单位： | 1. 华南理工大学轻工与食品学院,广州,510640
2. 华南理工大学生物科学与工程学院,广州,510006 |
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| 摘 要： | 以精炼菜籽油为底物,通过酶法甘油解制备甘油二酯,比较了3种常用固定化脂肪酶甘油解制备甘油二酯的能力。结果表明:LipozymeRM IM具有较好的甘油耐受性,采用甘油预吸附的方式进行甘油解反应,可明显减少反应中酶活损失,显著提高酶的重复使用寿命。在菜籽油与甘油摩尔比1∶1,酶添加量为油质量的5%,硅胶与甘油质量比1∶1,反应温度60℃的优化条件下,甘油解反应8 h后,产物中的甘油二酯含量达到57.5%。通过硅胶预吸附甘油可以使LipozymeRM IM酶的多批次操作稳定性得到很大提高,半衰期达到22次,有应用于工业生产的潜力。
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| 关 键 词： | 甘油二酯 菜籽油 脂肪酶 甘油解 |
Synthesis of diacylglycerol by enzymatic glycerolysis |
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| WANG Weifei
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NING Zhengxiang
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XU Yang
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WANG Yonghua
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YANG Bo.Synthesis of diacylglycerol by enzymatic glycerolysis[J].China Oils and Fats,2012,37(5):31-34. |
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| Authors: | WANG Weifei NING Zhengxiang XU Yang WANG Yonghua YANG Bo |
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| Affiliation: | 1.College of Light Industry and Food Sciences,South China University of Technology, Guangzhou 510640,China;2.School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006,China) |
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| Abstract: | Diacylglycerol was synthesized by enzymatic glycerolysis using refined rapeseed oil as substrate.Three commercial immobilized lipases for preparing diacylglycerol were compared.The results showed that LipozymeRM IM had a good glycerol tolerability.Useful life of the lipase could be remarkably improved using glycerol preadsorbed by silica gel.Diacylglycerol content of the product was 57.5% after 8 h reaction under the optimum conditions: molar ratio of rapeseed oil to glycerol 1∶ 1,enzyme dosage 5%(based on the mass of rapeseed oil),mass ratio of silica gel to glycerol 1∶ 1,reaction temperature 60 ℃.The enzyme could be used 22 times repeatedly in batch process,which had a potential for industrial production. |
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| Keywords: | diacylglycerol rapeseed oil lipase glycerolysis |
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