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| 植物油中甾醇烯类物质的检测研究 | |
| 引用本文： | 于瑞祥,杨瑞钰,方晓明,赵宇翔,陈迪,丁卓平.植物油中甾醇烯类物质的检测研究[J].中国油脂,2012,37(6):81-85. |
| 作者姓名： | 于瑞祥 杨瑞钰 方晓明 赵宇翔 陈迪 丁卓平 |
| 作者单位： | 1. 上海海洋大学食品学院,上海,201306 2. 上海市标准化研究院,上海,200031 3. 上海出入境检验检疫局,上海,200135 4. 上海市食品药品监督所,上海,200030 |
| 基金项目： | 上海市食品安全地方标准制定项目，上海市教育委员会重点学科建设项目 |
| 摘 要： | 建立了GC-MS测定植物油中4种甾醇烯类物质的方法。样品用石油醚提取,经硅胶柱净化,HP-5毛细管柱(5%苯基聚硅氧烷,30 m)分离,质谱检测采用SIM模式。在0.062 5～1.0 mg/L的质量浓度范围内,峰面积与质量浓度呈良好线性,线性相关系数(r)大于0.99。在特级初榨橄榄油中添加0.025、0.050 mg/kg和0.100 mg/kg水平的甾醇烯类物质,平均加标回收率为78.6%～107.3%,相对标准偏差小于8%,方法检测限可达0.010 mg/kg。本方法准确、灵敏、可靠,已应用于实际样品分析,并可作为鉴别地沟油的方法之一。 |
| 关 键 词： | 气相色谱 质谱 甾醇烯类 植物油 地沟油 |
Determination of steradienes in vegetable oils |
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| YU Ruixiang , YANG Ruiyu , FANG Xiaoming , ZHAO Yuxiang , CHEN Di , DING Zhuoping.Determination of steradienes in vegetable oils[J].China Oils and Fats,2012,37(6):81-85. | |
| Authors: | YU Ruixiang YANG Ruiyu FANG Xiaoming ZHAO Yuxiang CHEN Di DING Zhuoping |
| Affiliation: | 1(1.College of Food Science & Technology,Shanghai Ocean University,Shanghai 201306,China; 2.Shanghai Institute of Standardization,Shanghai 200031,China; 3.Shanghai Exit-Entry Inspection and Quarantine Bureau,Shanghai 200135,China; 4.Shanghai Institute of Food and Drug Supervision,Shanghai 200030,China) |
| Abstract: | A method for the determination of 4 steradienes in vegetable oils by GC-MS was established.Vegetable oil sample was extracted by petroleum ether,cleaned up by silica column,separated by HP-5 column(5% phenyl polysiloxane,30 m) and determined by mass spectrometry with SIM mode.The mass concentrations of steradienes was between 0.062 5 mg/L and 1.0 mg/L,the calibration curve had good linear with correlation coefficients higher than 0.99.Extra virgin olive oils were added steradienes 0.025,0.050 mg/kg and 0.100 mg/kg,the average recovery ranged from 78.6% to 107.3%,and the relative standard deviations(RSD) were less than 8%.The limits of detection could achieve 0.010 mg/kg.This method was precise,sensitive and reliable,which had been applied to the practical sample analysis,and could be one of the methods to identify hogwash oil. |
| Keywords: | gas chromatography(GC) mass spectrometry(MS) steradiene vegetable oil hogwash oil |
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