预处理固定化脂肪酶催化合成生物柴油

探讨了预处理固定化Candida antarctica脂肪酶催化餐饮废油合成生物柴油的过程。将固定化Candida antarctica脂肪酶用叔丁醇处理3h后再用废油浸泡4h，用于催化酯交换反应，酯交换反应速率明显加快。研究发现，固定化Candida antarctica脂肪酶预处理后，过量甲醇对酶的抑制作用仍然存在。采用分步添加甲醇工艺，按总醇油摩尔比3：1，分别在0、0．5、1．0、1．5、2．0、2．5h等量加入甲醇，反应4h后，体系中甲酯含量达到97．86％，反应：效率是未处理固定化酶催化合成生物柴油体系的6倍。固定化酶重复使用仍具有较高活性。

Pretreatment of immobilized Candida antarctica lipase for biodiesel production from waste oil

The transesterification of waste oil to biodiesel catalyzed by pretreated immobilized Ca-ndida antarctica lipase was investigated.Transesterification progressed much faster when the enzyme was pre-incubated in tert-butanol for 3 h and subsequently in waste oil for 4 h.The inhibition of superfluous methanol still existed after the pretreatment of immobilized Candida antarctica lipase.The methyl ester content reached 97.86% within 4 h by stepwise addition of equivalent methanol at 0.5 h intervals from 0 to 2.5 h with the molar ratio of total methanol to oil 3:1.The efficiency of transesterification was 6 times faster than that of unpretreated immobilized lipase.The enzyme sho-wed no appreciable loss after being continuously operated.