| 3，5-二硝基水杨酸比色法测定烟草中水溶性总糖 |
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| 引用本文： | 罗志刚,曾满枝,凌晨.3，5-二硝基水杨酸比色法测定烟草中水溶性总糖[J].中国烟草科学,2000,21(2):34-36. |
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| 作者姓名： | 罗志刚 曾满枝 凌晨 |
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| 作者单位： | 华南农业大学!广州510642，华南农业大学!广州510642，华南农业大学!广州510642 |
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| 摘 要： | 研究了还原糖对３，５－二硝基水杨酸的还原产物的吸收光谱。结果表明，还原产物对波长４７４ｎｍ的光有最大吸收。找出了显色剂的最佳用量。作了标准曲线，同时对３个烟样进行了测定，并与经典方法的测定结果比较，相对误差为２－５％。
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| 关 键 词： | 烟草 水溶性总糖 比色法 水杨酸 标准曲线 测定 |
| 修稿时间： | 1999-05-18 |
Colorimetric
determination of water -soluble total sugar in tobacco with 3,5-dinitrosalicylic acid |
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| Luo Zhigang,Zeng Manzhi,Ling Chen,Lin Wenbo,Gong Zhiping,Yan Liwen.Colorimetric
determination of water -soluble total sugar in tobacco with 3,5-dinitrosalicylic acid[J].Chinese Tobacco Science,2000,21(2):34-36. |
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| Authors: | Luo Zhigang Zeng Manzhi Ling Chen Lin Wenbo Gong Zhiping Yan Liwen |
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| Affiliation: | South China Agricultural University Guangzhou 510642 |
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| Abstract: | The absorption spectrum of 3,5 dinitrosalicylic acid reducing product with reducing sugar has been studied.The result shows a maximum absorption at 474 nm,and gives the best suitable amount of the color reagent and the standard curve.When three tobacco samples are determined,the relative errors of the results obtained by this method related to those obtained by the classical method are in the range of 2% 5% |
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| Keywords: | Tobacco Water soluble total sugar Colorimetry 3 5 dinitrosalicylic acid Absorption spectrum Standard curve |
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