百合多糖的纯化及其对肠道菌群 失调小鼠的调节作用

对百合多糖进行纯化并探究其对肠道菌群失调小鼠的调节作用。以产率、脱色率、多糖含量为指标,比较AB-8、D315、D101、HPD-100四种大孔树脂对百合多糖的纯化效果;实验分为空白组(生理盐水10 mg/kg)、模型组(盐酸林可霉素10 mg/kg)、阳性组(丽珠肠乐10 mg/kg)、百合多糖低、中、高(50、100、200 mg/kg)剂量组;以脂多糖(LPS)、血清炎症因子IL-6、TNF-α及回肠组织SIgA的含量、菌群数量为指标,评价纯化后的百合多糖对肠道菌群失调小鼠的调节作用。结果表明:D315大孔树脂纯化效果最佳,纯化后百合多糖的产率、脱色率、糖含量分别为40.82%±1.28%、35.11%±1.12%、82.56%±1.18%;药理实验结果表明:百合多糖高剂量组(200 mg/kg)效果最佳。与模型组相比,高剂量组小鼠LPS、血清炎症因子IL-6、TNF-α的含量极显著降低(P<0.01),回肠组织SIgA含量极显著增加(P<0.01),肠杆菌、肠球菌的数量显著降低(P<0.05),乳酸杆菌、双歧杆菌的数量显著增加(P<0.05)。说明百合多糖对小鼠肠道菌群紊乱具有调节作用。

Purification of Lily Polysaccharide and Its Regulatory Effect on Intestinal Flora Dysregulation Mice

The lily polysaccharide was purified and explored for its regulation of intestinal dysregulated mice. The purification effects of four macroporous resins AB-8,D315,D101 and HPD-100 on lily polysaccharides were compared by the yields,decolorization rates and polysaccharide contents. The experiment was divided into blank group(saline 10 mg/kg),model group(lincomycin hydrochloride 10 mg/kg),positive group(lizhu intestine 10 mg/kg),and the low,medium and high(50,100,200 mg/kg)dose groups of lily polysaccharide. The mouse ileal tissue SIgA,plasma LPS,serum inflammatory factor IL-6,the contents of TNF-α and the number of microflora were used as indicators to evaluate the regulation effect of purified lily polysaccharide on intestinal flora imbalance mice. The results showed that the yields,decolorization rates and polysaccharide content of lily polysaccharide purified by D315 macroporous resin were the highest with 40.82%±1.28%,35.11%±1.12% and 82.56%±1.18%,respectively. The results of pharmacological experiments showed that the high dose group of lily polysaccharide had the best effect. Compared with the model group,the LPS contents,serum inflammatory factors IL-6 and TNF-α levels in the high dose group were significantly(P<0.01)decreased,and the ileal tissue SIgA contents was significantly increased(P<0.01),the number of Enterobacteriaceae and Enterococcus were significantly reduced(P<0.05),while the number of Lactobacillus and Bifidobacteria were increased(P<0.05). It indicated that lily polysaccharide had a regulatory effect on the intestinal flora disorder in mice.