农大4号’欧李果实花色苷对D-半乳糖致衰老小鼠保护作用研究

为了研究'农大4号'欧李果实花色苷对衰老小鼠保护作用,采用颈部皮下注射D-半乳糖构建衰老小鼠模型,用不同剂量(高、中、低剂量分别为500、250、125 mg·(kg·d)-1)的花色苷灌胃小鼠,计算小鼠心脏、肝脏、脾脏、肺脏及肾脏指数,并测定小鼠血清、心脏、肝脏、脾脏和肾脏中总抗氧化能力(total antioxidant capacity,T-AOC)、过氧化氢酶(catalase,CAT)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的指标变化情况。结果表明:与空白组相比,模型组小鼠肝脏指数、脾脏指数和肾脏指数显著下降(P<0.05),血清和各脏器组织中的T-AOC、SOD、GSH-Px、CAT均显著降低(P<0.05),MDA含量显著升高(P<0.05),说明衰老小鼠模型构建成功。与模型组相比,高剂量组的肝脏指数、脾脏指数和肾脏指数显著升高(P<0.05),血清和各脏器中的T-AOC、SOD、GSH-Px、CAT均有显著升高(P<0.05),MDA含量显著降低(P<0.05)。由此得出,'农大4号'欧李果实花色能有效增强小鼠的抗氧化能力,对衰老小鼠有较强的保护作用。

Protection Effect of Anthocyanins from the Chinese Dwarf Cherry ‘Nongda No 4’ on Aging Mouse by D-galactose

In order to study the effect of anthocyanin from the Chinese dwarf cherry ‘Nongda No 4’ on the protection of aging mouse,a mouse model of aging was established by subcutaneously injecting D-galactose in the neck and back,and the aging mice were gavaged with different doses(500,250,125 mg·(kg·d)-1)of anthocyanins. The heart,liver,spleen,lung and kidney indexes and the contents of malondialdehyde(MDA)and the activities of total antioxidant capacity(T-AOC),catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)in the heart,liver,spleen,kidney and serum of mice were assessed. The results showed that compared with the control group,the liver,spleen and kidney indexes of mice in the model group decreased significantly(P<0.05),T-AOC,SOD,GSH-Px,and CAT activities in the serum and organs decreased significantly(P<0.05),and MDA content increased significantly(P<0.05),indicating successful construction of aging mice model. Compared with the model group,the liver,spleen and kidney indexes and T-AOC,SOD,GSH-Px,and CAT activities in the serum and organ tissues were increased significantly(P<0.05)and MDA content was decreased significantly(P<0.05)of mice in the high dose. Therefore,anthocyanins from the Chinese dwarf cherry ‘Nongda No 4’ can effectively improve the antioxidant ability and protection ability in mice.