黄芪多糖对果蝇寿命和抗氧化作用的影响

为探讨黄芪多糖对果蝇寿命和抗氧化的影响,将含有0.1、0.5、1.0、1.5、2.0 μg/mL的黄芪多糖培养基喂食果蝇,通过生存实验研究其寿命,通过逆重力爬行能力实验研究其运动能力,通过抗氧化活性实验测定其SOD、T-AOC和MDA活性。结果表明,当黄芪多糖浓度为1.0 μg/mL时,与对照相比,雌、雄性果蝇的平均寿命分别延长了27.69%和25.77%,半数死亡时间分别延长了52.09%和53.65%,最高寿命分别延长了16.71%和15.78%,均显著高于对照(P<0.05);雌、雄果蝇的逆重力爬行能力最强,分别增加了14.16%和27.97%,均显著高于对照(P<0.05)。雌、雄果蝇体内T-AOC和SOD酶活最高,而MDA含量最少。因此,黄芪多糖可显著提高果蝇的寿命、机体的运动能力和抗氧化能力。

Effects of Astragalus Polysaccharide on Life Span and Antioxidation of Drosophila melanogaster

In order to explore the effect of Astragalus polysaccharide on the life span and antioxidation of D. melanogaster,different concentrations of Astragalus polysaccharide(0.1,0.5,1.0,1.5,2.0 μg/mL)were fed to D. melanogaster. Life span was studied through survival experiment,exercise capacity was studied through negative geotaxis capability experiment,and SOD,T-AOC and MDA activities were determined through antioxidant activity experiment. The results showed that,when Astragalus polysaccharide concentration was 1.0 μg/mL,the average life span of female and male D. melanogaster were prolonged by 27.69% and 25.77% compared with the control. Half death time was prolonged by 52.09% and 53.65%,respectively. The maximum life span was extended by 16.71% and 15.78% respectively,which were significantly higher than the control(P<0.05).Female and male D. melanogaster had the strongest ability to negative geotaxis capability,increased by 14.16% and 27.97% respectively,which were significantly higher than the control(P<0.05). In male and female D. melanogaster,T-AOC and SOD activities were the highest,while MDA contents was the lowest. Astragalus polysaccharide could significantly improve life span,exercise capacity and antioxidant capacity of D. melanogaster.