牡蛎低聚肽对CTX诱导的PADAM大鼠性功能及生殖功能的干预作用

目的:探讨不同剂量的牡蛎低聚肽(OOP)对男性雄激素部分缺乏综合征(PADAM)大鼠性功能和生殖功能的干预作用。方法:将雄性SD大鼠随机分为正常对照组(NC组)、PADAM组(PC)、PADAM+低、中和高剂量OOP组(PLO、PMO、PHO)5组。除NC组外,其余大鼠在利用环磷酰胺诱导PADAM形成的同时补充不同剂量的OOP,6周后测定血清睾酮(TT)、游离睾酮(FT)、黄体生成素(LH)、卵泡刺激素(FSH)和睾丸雄激素受体(AR)含量、捕捉潜伏期、交配潜伏期、捕捉次数、交配次数以及附睾中的精子数量、活率和活力。结果:与NC组相比,PC组血清TT、FT、LH含量、交配次数以及精子数量、活率和活力均显著降低(P<0.05),捕捉潜伏期和交配潜伏期显著延长(P<0.05)。与PC组相比,PLO组捕捉潜伏期显著缩短(P<0.01)、捕捉和交配次数显著增多(P<0.01);除PMO组精子数量无显著性增加外(P>0.05),PMO和PHO组血清TT、FT、FSH、LH和睾丸AR含量以及捕捉和交配次数、精子数量、活率和活力显著增加(P<0.05),捕捉和交配潜伏期显著缩短(P<0.05),且PMO和PHO组血清TT、FSH含量和交配次数以及PHO组血清LH和睾丸AR含量显著高于PLO组(P<0.05)。结论:补充牡蛎低聚肽能够通过改善HPG轴有效防止CTX引起的PADAM的形成,显著改善性功能和生殖功能,并具有一定的剂量效应,以中、高剂量的效果最佳。

Intervention of Oyster Oligopeptides on Sexual and Reproductive Functions in PADAM Rats Induced by CTX

Objective:To investigate the effects of oyster oligopeptides(OOP)at different doses on sexual and reproductive functions in male partial androgen deficiency syndrome(PADAM)rats. Methods:Male SD rats were randomly divided into five groups:Normal control group(NC group),PADAM control group(PC),PADAM+low,medium and high dose OOP group(PLO,PMO,PHO). In addition to NC group,the other rats were supplemented with OOP at different doses while PADAM was induced by cyclophosphamide(CTX). Serum testosterone(TT),free testosterone(FT),luteinizing hormone(LH),follicle stimulating hormone(FSH)and testicular androgen receptor(AR),capture latency,mating latency and number of catches and mating,sperm quantity,viability and vitality in epididymis were measured 6 weeks later. Results:Compared with NC group,the contents of serum TT,FT,LH,the mating times and the number,viability and vitality of sperm in PC group significantly decreased(P<0.05),the capture latency and mating latency significantly increased(P<0.05). Compared with PC group,the capture latency was significantly shortened(P<0.01),and the times of capture and mating in PLO group were significantly increased(P<0.01). Except for no significant increase of sperm number in PMO group(P>0.05),the contents of serum TT,FT,FSH,LH,testicular AR and the times of capture and mating,the sperm number,viability and motility in the PMO and PHO groups increased significantly(P<0.05),the latency of capture and mating was significantly shortened(P<0.05). And the serum TT,FSH contents and mating times in PMO and PHO groups as well as the contents of serum LH and testicular AR in PHO group were significantly higher than those in PLO group(P<0.05). Conclusion:Oral oyster oligopeptides can effectively prevent the formation of PADAM induced by CTX through improving HPG axis,significantly improve sexual and reproductive functions,and has a certain dose effect,with the best effect of medium and high dose.