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响应面法优化黄浆水絮凝工艺及其代谢组学分析
引用本文:王民申,李勇,冯进,李春阳,侯万伟,Oluwole Oluwatoyin Bolanle,Chuon Mony Roth.响应面法优化黄浆水絮凝工艺及其代谢组学分析[J].食品工业科技,2022,43(1):244-252.
作者姓名:王民申  李勇  冯进  李春阳  侯万伟  Oluwole Oluwatoyin Bolanle  Chuon Mony Roth
作者单位:1.哈尔滨商业大学食品工程学院, 黑龙江哈尔滨 1500762.江苏省农业科学院农产品加工研究所, 江苏南京 2100143.江苏省农业科学院农业资源与环境研究所, 江苏南京 2100144.青海省农林科学院, 青海西宁 8100005.奥肖迪联邦工业研究所食品技术部, 尼日利亚拉各斯 9990626.柬埔寨农业渔业部农产品和食品实验室, 柬埔寨金边 10103
基金项目:2017年青海省科技计划项目科技合作专项(2017-HZ-816)。
摘    要:为了提高黄浆水的附加值和利用率,缓解排放黄浆水带来的环境污染,本文对黄浆水中的可溶性固形物进行絮凝处理。在单因素实验基础上结合响应面法对黄浆水中可溶性固形物的絮凝工艺进行优化,并且对絮凝前后黄浆水进行了蛋白质和总糖含量测定、生化需氧量和化学需氧量测定以及代谢组学测定。实验结果表明,絮凝沉降黄浆水可溶性固形物最佳工艺参数为:壳聚糖添加量为0.54 mg/mL,海藻酸钠添加量为0.24 mg/mL,pH为4.4,温度为47 ℃,此时黄浆水可溶性固形物沉降率为(46.26%±0.38%);蛋白质含量下降40.68%、总糖含量下降8.41%;生化需氧量和化学需氧量去除率分别为43%、40.95%。代谢组学实验结果表明:黄浆水絮凝前后有66种化合物含量存在显著变化。本研究结果表明絮凝能有效的降低黄浆水中可溶性固形物的含量,为黄浆水的进一步开发利用提供一定的参考。

关 键 词:黄浆水    响应面分析    可溶性固形物    絮凝法    代谢组学
收稿时间:2021-08-09

Optimization of Flocculation Process of Yellow Serofluid by Response Surface Methodology and Its Metabolomics Analysis
WANG Minshen,LI Yong,FENG Jin,LI Chunyang,HOU Wanwei,OLUWOLE Oluwatoyin Bolanle,CHUON Mony Roth.Optimization of Flocculation Process of Yellow Serofluid by Response Surface Methodology and Its Metabolomics Analysis[J].Science and Technology of Food Industry,2022,43(1):244-252.
Authors:WANG Minshen  LI Yong  FENG Jin  LI Chunyang  HOU Wanwei  OLUWOLE Oluwatoyin Bolanle  CHUON Mony Roth
Affiliation:(College of Food Engineering,Harbin University of Commerce,Harbin 150076,China;Institute of Agro-Product Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Institute of Agricultural Resources and Environment,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Qinghai Academy of Agricultural and Forestry Sciences,Xining 810000,China;Food Technology Department,Oshodi Federal Institute Industrial Research,Lagos 999062,Nigeria;Cambodia Laboratory of Agricultural Products and Foods,Ministry of Agriculture Forestry and Fisheries of Cambodia,Phnom Penh 10103,Cambodia)
Abstract:In order to improve the added value and utilization rate of yellow serofluid and alleviate the environmental pollution caused by the discharge of yellow serofluid, the soluble solids in yellow serofluid were flocculated. On the basis of single factor experiment, the flocculation process of soluble solids in yellow serofluid was optimized with response surface methodology. The contents of protein and total sugar, BOD (biochemical oxygen demand), COD (chemical oxygen demand) and metabolomics of yellow serofluid before and after flocculation were determined. The experimental results showed that the optimal technological parameters of flocculation sedimentation of soluble solids in yellow serofluid were as follows: Chitosan content was 0.54 mg/mL, sodium alginate content was 0.24 mg/mL, pH was 4.4, temperature was 47 ℃, and the sedimentation rate of soluble solids in yellow serofluid was (46.26%±0.38%). Protein content decreased by 40.68% and total sugar content decreased by 8.41%. The removal rates of BOD and COD were 43% and 40.95%, respectively. Metabolomics experiment results showed that 66 compounds in yellow serofluid were significantly changed before and after flocculation. The results showed that flocculation could effectively reduce the content of soluble solids in yellow serofluid, which provides a certain reference for further development and utilization of yellow serofluid.
Keywords:yellow serofluid  response surface methodology  soluble solids  flocculation method  metabolomics
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