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表没食子儿茶素没食子酸酯的提取分离工艺研究
引用本文:郭秋韵,康丽红,李露,徐昆,张学俊.表没食子儿茶素没食子酸酯的提取分离工艺研究[J].食品工业科技,2011(10):358-360,363.
作者姓名:郭秋韵  康丽红  李露  徐昆  张学俊
作者单位:中北大学理学院,山西太原,030051
摘    要:采用无水乙醇对茶叶进行浸提,用乙酸乙酯和蒸馏水分别对浸提液进行萃取后浓缩包含表没食子儿茶素没食子酸酯epigallocatechin gallate,(-)-EGCG]在内的黄酮类物质。利用聚酰胺装填的层析柱对茶叶中的(-)-EGCG进行分离,并对分离产品结果进行分析。本实验以活性炭为脱色剂,考察了活性炭的用量、脱色时间、脱色次数、脱色后溶液pH、乙酸乙酯与水的配比以及洗脱荆中乙醇浓度等条件对实验结果产生的影响。在各项条件最优的情况下,每10g绿茶可以提取出纯度大于98%的(-)-EGCG单体0.39g。

关 键 词:表没食子儿茶素没食子酸酯  聚酰胺  活性炭  纯度  产率

Study on extraction and separation of epigallocatechin gallate
GUO Qiu-yun,KANG Li-hong,LI Lu,XU Kun,ZHANG Xue-jun.Study on extraction and separation of epigallocatechin gallate[J].Science and Technology of Food Industry,2011(10):358-360,363.
Authors:GUO Qiu-yun  KANG Li-hong  LI Lu  XU Kun  ZHANG Xue-jun
Affiliation:GUO Qiu-yun,KANG Li-hong,LI Lu,XU Kun,ZHANG Xue-jun(Department of Science,North University of China,Taiyuan 030051,China)
Abstract:Using anhydrous alcohol to extract of tea,and using ethyl acetate and distilled water to soak mention fluid respectively to extract enrichment flavonoids substances which contains epigallocatechin gallate.Then using polyamide chromatography column in the(-) -EGCG separation,and analysis the results. The active carbon was used as decoloring agents,at the same time to examine the dosage of activated carbon,decoloring time and decoloring number,the pH of bleaching solution,the proportion of ethyl acetate and w...
Keywords:epigallocatechin gallate  polyamide  activate carbon  purity  yield  
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