白燕与血燕的营养成分分析和比较

为分析和比较白燕与血燕的营养成分,分别采用凯氏定氮法、邻苯二甲醛-氯甲酸芴甲酯（OPA-FMOC）柱前衍生反相高效液相色谱法、柱前衍生二极管阵列/荧光检测器串联反相高效液相色潽法和电感耦合等离子体质谱法（ICP-MS）等方法测定了白燕与血燕的蛋白质含量、氨基酸组成及比例、唾液酸含量、无机元素含量。结果表明:白燕蛋白质含量为56.5%,氨基酸总量49.22%,其中必需氨基酸占氨基酸总量的58.0%,唾液酸含量占干重15.3%。血燕蛋白质含量为55.0%,氨基酸总量44.16%,必需氨基酸占氨基酸总量的57.9%,唾液酸含量约为13.3%。两种燕窝中蛋白质、氨基酸含量均较高,必需氨基酸占氨基酸总量50%以上,唾液酸含量均大于10%,无机元素种类丰富。白燕的蛋白质含量、氨基酸总量、唾液酸含量比血燕略高,但两者不存在明显差异,两种燕窝中无机元素含量略有差异。该结果为燕窝产品的深入研究提供了重要依据和实验思路。 

Comparative analysis of nutritional components in White-edible bird's nest (EBN) and Red-EBN

To compare the nutritional components in White-EBN and Red-EBN,protein was determined by using Kjeldahl method,precolumn derivatization with o-phthaldialdehyde(OPA) and fluorenylmethyl chloroformate(FMOC chloride) was used on the determination of amino acids,pre-column derivation reversed phase high performance liquid chromatography with photodiode array detector(DAD) and fluorescence detector(FLD) method was used for the determination of N-acetylneuraminic acid,the content of inorganic elements was studied...