超声波酸水解法提取豆渣中异黄酮条件优化

目的:对水酶法提取大豆油后的副产物进行研究,提取其生理活性物质大豆异黄酮。方法:采用超声方法和酸水解方法相结合对水酶法提取大豆油副产物进行异黄酮提取,在此基础上进行响应面优化,确定最佳提取工艺。结果:分析了影响异黄酮提取的各种因素,并予以优化。优化后工艺条件为:料液比为1:12.54,乙醇浓度为70.28%,盐酸浓度为2.6mol/L,水解提取时间为30min,提取温度为30℃。结论:超声波和酸水解的方法适用于水酶法提取油后豆渣中大豆异黄酮的提取,在加入酸水解后大豆异黄酮总提取量较单纯70%乙醇提取法提高42.55%。

Optimization of soybean isoflavone extaraction from okara by ultrasonic and acid hydrolysis

Objective:In this research,the by-products obtained after extracting oil by enzymatic hydrolysis was studied,aiming at recycling the resources and producing isoflavones.Results:The optimal technological conditions of extracting isoflavone from oil by-products using enzymatic hydrolysis were determined:material fluid ratio was 1:12.54,alcohol concentration was 70.28%,hydrochloric acid concentration was 2.6mol/L,hydration time was 30min,hydration temperature was 30℃.Conclusions:The method of ultrasonic and acid hydrolysis was applied for soybean isoflavone extaraction from okara of enzymatic hydrolysis.The content of soybean isoflavone with acid hydrolysis was 42.55% higher than that with 70% ethanol extraction.