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| 高效液相色谱—二极管阵列检测法测定原料乳中的舒巴坦 | |
| 引用本文： | 刘珊珊,单艺,满朝新,谢鲲昊,卢雁,胡惠秩,阎天文,姜毓君.高效液相色谱—二极管阵列检测法测定原料乳中的舒巴坦[J].食品工业科技,2012,33(16):64-66. |
| 作者姓名： | 刘珊珊 单艺 满朝新 谢鲲昊 卢雁 胡惠秩 阎天文 姜毓君 |
| 作者单位： | 1. 东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨150030 2. 国家乳业工程技术研究中心,黑龙江哈尔滨,150086 3. 东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨150030 国家乳业工程技术研究中心,黑龙江哈尔滨150086 |
| 基金项目： | 国家科技支撑计划课题(2012BAK17B04);国家科技支撑计划课题(2009BADB9B06) |
| 摘 要： | 建立了利用高效液相色谱-二极管阵列法(high-performance liquid chromatography-photodiode array,HPLC-PDA)测定原料乳中舒巴坦含量的方法。采用C18色谱柱,以5.44g/L磷酸二氢钾溶液(pH5.0)-乙腈(95∶5)为流动相,流速为lmL/min,检测波长220nm,柱温30℃。样品经乙腈提取、乙酸锌沉淀蛋白,HPLC-PDA检测。舒巴坦浓度在10～200μg/mL范围内,线性良好(R2=0.9998),添加回收率为98.82%～101.42%,检出限为0.25mg/kg,定量限为0.75mg/kg。该方法操作简单,结果准确、可靠,适用于原料乳中舒巴坦的快速检测。 |
| 关 键 词： | 舒巴坦 高效液相色谱-二极管阵列检测法 含量测定 原料乳 |
Determination of sulbactam in raw milk by the high-performance liquid chromatography-photodiode array detection |
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| LIU Shan-shan,SHAN Yi,MAN Chao-xin,XIE Kun-hao,LU Yan,HU Hui-zhi,YAN Tian-wen,JIANG Yu-jun.Determination of sulbactam in raw milk by the high-performance liquid chromatography-photodiode array detection[J].Science and Technology of Food Industry,2012,33(16):64-66. | |
| Authors: | LIU Shan-shan SHAN Yi MAN Chao-xin XIE Kun-hao LU Yan HU Hui-zhi YAN Tian-wen JIANG Yu-jun |
| Affiliation: | 1,2,* (1.Key Laboratory of Dairy Science,Ministry of Education,Department of Food Science, Northeast Agricultural University,Harbin 150030,China; 2.National Research Center of Dairy Engineering and Technology,Northeast Agricultural University, Harbin 150086,China) |
| Abstract: | To develop a method for the determination of sulbactam in raw milk using high performance liquid chromatography-photodiode array detector(HPLC-PDA). C18 column was used,the mobile phase consisted of 5.44g/L potassium dihydrogen phosphate solution(pH5.0)-acetonitrile(95∶5) at the flow rate of 1mL/min,the detection wavelength was 220nm.The column temperature was 30℃. Acetonitrile was used for extraction,zinc acetate was used for protein precipitation,the filtered fluid was determined by high performance liquid chromatography-photodiode array detector. The linear calibration curve was obtained in the concentration range of 1～200μg/mL(R2=0.9998) with the lower limit of detection of 0.25mg/kg and lower limit quantification of 0.75mg/kg. The average recovery of sulbactam was 98.82%～101.42%. The method was simple,accurate,reliable and could be used for rapid determination of sulbactam in raw milk. |
| Keywords: | sulbactam high-performance liquid chromatography-photodiode array(HPLC-PDA) detection content measured raw milk |
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