| 纳豆芽孢杆菌液态发酵生产γ-聚谷氨酸 |
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| 引用本文： | 朱丹,邹水洋,康建雄.纳豆芽孢杆菌液态发酵生产γ-聚谷氨酸[J].食品工业科技,2012,33(17):151-153,158. |
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| 作者姓名： | 朱丹 邹水洋 康建雄 |
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| 作者单位： | 1. 东莞理工学院化学与环境工程学院,广东东莞,523808
2. 华中科技大学环境科学与工程学院,湖北武汉,430074 |
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| 基金项目： | 东莞市高等院校科研机构科技计划项目(200910814046) |
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| 摘 要： | 对纳豆芽孢杆菌CICC 20643液态发酵生产γ-聚谷氨酸(γ-PGA)的工艺进行了研究。采用单因素实验和正交实验获得了生产γ-PGA的优化培养条件:蔗糖25g/L,酵母膏5g/L,谷氨酸钠40g/L,装液量70mL/250mL锥形瓶,起始pH6.5,菌种在37℃、120r/min培养24h后,于培养基中添加5%NaCl,继续培养24h,γ-PGA的产量达到18.04g/L。实验结果表明:纳豆芽孢杆菌CICC20643是一株产γ-PGA优良菌种,在发酵过程中添加NaCl的工艺能明显提高γ-PGA的产量。
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| 关 键 词： | 纳豆芽孢杆菌 γ-聚谷氨酸 液态发酵 氯化钠 谷氨酸 |
Production of poly-γ-glutamic acid by submerged fermentation with Bacillus subtilis natto |
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| ZHU Dan,ZOU Shui-yang,KANG Jian-xiong.Production of poly-γ-glutamic acid by submerged fermentation with Bacillus subtilis natto[J].Science and Technology of Food Industry,2012,33(17):151-153,158. |
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| Authors: | ZHU Dan ZOU Shui-yang KANG Jian-xiong |
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| Affiliation: | 1.School of Chemistry and Environmental Engineering,Dongguan University of Technology,Dongguan 523808,China;2.School of Environmental Science and Engineering,Huazhong University of Science and Technology,Wuhan 430074,China) |
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| Abstract: | Production of poly-γ-glutamic acid(γ-PGA)by cultivation of Bacillus subtilis natto CICC 20643 in submerged state had been studied.The optimum cultivation conditions through single factor and orthogonal experiments were set as follows:sucrose 25g/L,yeast extract 5g/L,glutamic acid 40g/L,initial pH at 6.5,culture volume 70mL in 250mL conical flask.Under the optimum cultivation conditions,B.subtilis natto was shaking cultivated at 37℃,120r/min for 24h,then 5%NaCl was added,and subsequently cultivated for another 24h.As a result,the yield ofγ-PGA was got at 18.04g/L.The experimental result showed that B.subtilis natto CICC 20643 was a strain of high-yieldγ-PGA and addition of NaCl in the course of cultivation could increase the yield ofγ-PGA. |
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| Keywords: | Bacillus subtilis natto γ-PGA submerged fermentation NaCl glutamic acid |
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