荷叶活性物质超声、微波辅助提取比较及人工胃液处理对其抗氧化活性的影响

为了提高荷叶抗氧化活性物质的提取效率及探索体内胃环境消化对荷叶提取物抗氧化活性的影响,本实验采用测定总还原力、总抗氧化力、羟自由基及DPPH自由基清除率的方法对超声和微波辅助提取荷叶抗氧化活性物质的效率进行了比较,并采用人工胃液在体外模拟体内胃环境对荷叶粗提物进行了处理。结果表明,微波辅助提取所得提取液的抗氧化活性(总还原力、总抗氧化力、羟自由基及DPPH自由基清除率)强于超声辅助提取液,其总黄酮和总多酚含量也高于超声辅助提取液;荷叶粗提物经人工胃液处理后,其抗氧化活性(总抗氧化力、羟自由基、超氧阴离子及DPPH自由基清除率)显著增强。

Comparison of ultrasound and microwave-assisted extractions of bioactive components from lotus leaf and effect of the artificial gastric juice immersion on the antioxidant activities

In order to improve the extraction efficiency of lotus leaf antioxidants and explore the effect of artificial gastric juice immersion on the antioxidant activities,the extraction efficiency of lotus leaf antioxidants of ultrasound and microwave-assisted extractions by determining reducing power,total antioxidant power,scavenging percentage of hydroxyl radicals,DPPH radicals and the effect of the artificial gastric juice immersion on the antioxidant activities were studied.The results showed that the antioxidant activities(the reducing power,total antioxidant power,scavenging percentages of superoxide anion radicals and DPPH radicals)of the microwave-assisted extracts were stronger than those of ultrasound assisted extracts,the total flavonoids and polyphenols contents of the microwave-assisted extracts were higher than those of ultrasound assisted extracts.The antioxidant activities(the total antioxidant power,scavenging percentages of superoxide anion radicals,hydroxyl radicals and DPPH radicals)of lotus crude extracts were much improved after immersed by the artificial gastric juice.