响应面法优化碱蓬根总生物碱的提取工艺及其抑菌活性

为优化碱蓬根总生物碱的提取工艺,并研究其抑菌活性,以碱蓬根为原料,在单因素实验基础上,采用响应面法确定碱蓬根总生物碱的最佳提取条件,管碟法及两倍稀释法检测碱蓬根总生物碱提取物对三种常见致病菌(大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌)的抑制效果。结果表明,当料液比为1:25 g/mL,乙醇浓度86%,提取温度78 ℃,提取时间2.1 h时,总生物碱得率最高为0.521%±0.04%。碱蓬根总生物碱对大肠杆菌及金黄色葡萄球菌有一定的抑菌效果,最低抑菌浓度为0.4 mg/mL,对枯草芽孢杆菌无抑菌性。该提取工艺高效可行,可用于碱蓬根中总生物碱的提取,同时提取物具有一定的抑菌性。

Optimization of the Extraction Technology of Total Alkaloids from the Roots of Suaeda salsa by Response Surface Method and Its Antibacterial Activity

To optimize the extraction process of total alkaloids from the roots of Suaeda salsa and study its antibacterial activity, based on the single-factor experiments, response surface method was used to determine the optimal extraction conditions of total alkaloids from the roots of Suaeda salsa. The cylinder-plate method and the two-dilution method were used to detect the inhibitory effects of the total alkaloid extract of the roots of Suaeda salsa on three common pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Bacillus subtilis). The results showed that the highest yield of total alkaloids was 0.521%±0.04% when the ratio of material to liquid was 1:25 g/mL, the concentration of ethanol was 86%, the extraction temperature was 78℃, and the extraction time was 2.1 h. The total alkaloid of the roots of Suaeda salsa had a certain bacteriostatic effect on Escherichia coli and Staphylococcus aureus. The minimum bacteriostatic concentration was 0.4 mg/mL, and it had no bacteriostatic effect on Bacillus subtilis. The extraction process was efficient and feasible, and could be used for the extraction of total alkaloids from the roots of Suaeda salsa, and the extracts had certain bacteriostatic properties.