马齿苋多糖对四氯化碳诱导的小鼠急性肝损伤的保护作用

目的:探讨马齿苋多糖对CCl₄诱导的小鼠急性肝损伤的保护作用。方法:采用CCl₄建立小鼠急性肝损伤模型。随机将60只小鼠分成正常组、模型组、联苯双酯组(200 mg/kg·d)、马齿苋多糖低、中、高剂量组(1.5、3、6 g/kg/d),连续给药7 d后,除正常组小鼠外,其他各组小鼠腹腔注射0.2%CCl₄花生油10 mL/kg;采用ELISA法检测血清中AST、ALT和ALP含量及肝组织匀浆液中MDA、SOD、GSH和GSH-Px活性,HE染色观察肝组织病理学变化,Western Blot检测肝脏组织细胞核内Nrf-2和HO-1蛋白的表达水平。结果:与模型组比较,马齿苋多糖低剂量组能够显著性降低血清中ALP含量(P<0.05),同时显著性升高肝组织匀浆液SOD水平及Nrf-2蛋白表达水平(P<0.05);马齿苋多糖中剂量组能够显著性降低肝脏指数、血清中AST、ALT含量及肝组织匀浆液中MDA水平(P<0.05),同时显著性升高肝匀浆液中GSH、GSH-Px水平(P<0.05),极显著性降低血清中ALP含量和升高肝组织匀浆液中SOD水平及Nrf-2和HO-1蛋白表达(P<0.01);马齿苋多糖高剂量组能够显著性降低肝脏指数和血清中AST含量(P<0.05),同时显著性升高肝组织匀浆液中GSH和GSH-Px水平(P<0.05),极显著性降低血清中AST、ALP、MDA含量(P<0.01),同时极显著性升高肝组织匀浆液中SOD水平及Nrf-2和HO-1蛋白表达水平(P<0.01);另外,肝组织病理学观察结果表明,马齿苋多糖可以减轻CCl₄对肝脏的病理损伤。结论:马齿苋多糖对CCl₄诱导的肝损伤有一定的保护作用,其机制与激活Nrf-2/HO-1信号通路有关。

Protective Effect of Purslane Polysaccharide on CCl4 Induced Acute Liver Injury in Mice

Objective:To investigate the protective effect of purslane polysaccharide on CCl₄ induced acute liver injury in mice. Methods:Establishment of acute liver injury model in mice by intraperitoneal injection of carbon tetrachloride(CCl₄). 60 mice were randomly divided into normal group,model group,biphenyl diester group(200 mg/kg·d),purslane polysaccharide low,medium and high dose group(1.5,3,6 g/kg/d),and were given continuous gavage for 7 days. In addition to the normal mice,other mice in each group were injected with 0.2% CCl₄ peanut oil 10 mL/kg intraperitoneally. Calculation of liver index,and the levels of AST,ALT and ALP in serum and the activities of MDA,SOD,GSH and GSH-Px in liver were measured by ELISA,HE staining to observe the pathological changes of liver tissue,and detection of Nrf-2 and HO-1 protein expression in liver by Western Blot. Results:Comparison with normal group,purslane polysaccharide low-dose group could significantly reduce the content of serum ALP(P<0.05). At the same time,SOD level and Nrf-2 protein expression level in liver homogenate were significantly increased(P<0.05). In the medium dose group,the liver index,AST and ALT contents in serum and MDA level in liver homogenate were significantly decreased(P<0.05),while the GSH and GSH-Px levels in liver homogenate were significantly increased(P<0.05),ALP content in serum and SOD level and Nrf-2 and HO-1 protein expression in liver homogenate were significantly increased(P<0.01). Purslane polysaccharide high-dose group could significantly reduce the content of AST in serum(P<0.05),liver index,significantly increase the levels of GSH and GSH-Px in liver tissue homogenate(P<0.05),significantly reduce the contents of AST,ALP and MDA in serum(P<0.01),and significantly increase the levels of SOD and Nrf-2 and HO-1 protein in liver tissue homogenate(P<0.01). In addition,the results of liver histopathology showed that purslane polysaccharide could reduce the pathological damage of liver caused by CCl₄. Conclusion:The protective effect of purslane polysaccharide on CCl₄ induced acute liver injury in mice is related to the activation of Nrf-2/HO-1 signal pathway.