| 薤白多糖的分离纯化及组成分析 |
| |
| 引用本文： | 夏新奎,杨海霞,李纯,周魏.薤白多糖的分离纯化及组成分析[J].食品工业科技,2010(1). |
| |
| 作者姓名： | 夏新奎 杨海霞 李纯 周魏 |
| |
| 作者单位： | 信阳农业高等专科学校生物技术系,河南信阳,464000 |
| |
| 基金项目： | 河南省科技攻关项目(991020207) |
| |
| 摘 要： | 采用DEAE-纤维素离子交换柱层析和SephadexG-150凝胶柱层析,对薤白粗多糖(PAM)进行了分离纯化;利用醋酸纤维素薄膜电泳和凝胶过滤法鉴定精制薤白多糖纯度;硅胶薄层层析分析精制多糖的单糖组成。结果表明:DEAE-纤维素柱分别用水、0.1mol.L-1NaCl、0.5mol.L-1Na0H洗脱分得三种级分,即水洗级分(PAM-I)、盐洗级分(PAM-II)和碱洗级分(PAM-III);这三种级分再经过SephadexG-150柱进一步分离纯化,得三个主要级分PAM-Ib、PAM-IIa及PAM-III’;经醋酸纤维素薄膜电泳和凝胶过滤法鉴定,它们为均一的多糖;硅胶薄层层析分析显示,PAM-Ib主要由半乳糖和葡萄糖组成,PAM-IIa主要由半乳糖、葡萄糖、果糖、木糖和鼠李糖组成,PAM-III’主要由半乳糖、葡萄糖和木糖组成。
|
| 关 键 词： | 薤白多糖 分离纯化 组成分析 |
Study on extraction, purification and component of polysaccharides from Allium macrosttemon Bge. |
| |
| XIA Xin-kui,YANG Hai-xia,LI Chun,ZHOU Wei.Study on extraction, purification and component of polysaccharides from Allium macrosttemon Bge.[J].Science and Technology of Food Industry,2010(1). |
| |
| Authors: | XIA Xin-kui YANG Hai-xia LI Chun ZHOU Wei |
| |
| Affiliation: | XIA Xin-kui,YANG Hai-xia,LI Chun,ZHOU Wei(Department of Biotechnology,Xinyang Agricultural College,Xinyang 464000,China) |
| |
| Abstract: | The polysaccharides from Allium macrosttemon Bge.were separated,purified by DEAE-cellulose chromatography and Sephadex G-150 gel chromatography.Their purity were tested by cellulose acetate pellicle electrophoresis and gel filtration on Sephadex G-150.Their component were measured by means of thin-layer chromatography(TLC).The result showed:PAM was separated into three fractions(PAM-I,PAM-II and PAM-III)by DEAE-cellulose chromatography.Then they were purified by Sephadex G-150 gel chromatography into PAM-Ib... |
| |
| Keywords: | Allium macrosttemon Bge polysaccharides extraction purification components analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
