| 产4-羟基-2,5-二甲基-3(2H)-呋喃酮酵母菌的分离、筛选及分子生物学鉴定 |
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| 引用本文： | 王鹏霄,程林坤,陈存社,吕跃钢,宋焕禄.产4-羟基-2,5-二甲基-3(2H)-呋喃酮酵母菌的分离、筛选及分子生物学鉴定[J].食品工业科技,2010(2). |
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| 作者姓名： | 王鹏霄 程林坤 陈存社 吕跃钢 宋焕禄 |
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| 作者单位： | 北京工商大学化学与环境工程学院,北京,100048 |
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| 基金项目： | 北京市高校人才强教计划学术创新人才项目资助(PHR200906110) |
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| 摘 要： | 以菠萝、草莓等水果为分离源,分离、筛选利用D-果糖产香料HDMF酵母菌株,并对其高产菌株进行分子生物学鉴定。采用稀释涂布法分离酵母菌株,高效液相色谱法检测HDMF产量,26SrDNAD1/D2区序列分析及系统发育分析鉴定菌株。共分离得到46株酵母,5株可利用D-果糖产HDMF;产量较高的两株菌为:C5(6.84mg/L)、P3(10.96mg/L),分别约为已报道毕赤氏酵母属(Pichia capsulata)利用L-(+)-鼠李糖产HDMF的3倍和5倍;26S rDNA D1/D2区序列分析及系统发育分析结果显示,P3与Pichia caribbica(毕赤酵母属),C5与Hanseniaspora sp.(有孢汉逊酵母属)相似性均在99%以上,分子生物学法鉴定P3为Pichia caribbica,C5为Hanseniaspora sp.
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| 关 键 词： | 酵母菌 微生物发酵 HDMF 26SrDNA |
Isolation,screening and molecular biological Characterization of yeasts producing 4-hydroxy-2,5-dimethyl-3(2H)-furanone(HDMF) |
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| WANG Peng-xiao,CHENG Lin-kun,CHEN Cun-she,LV Yue-gang,SONG Huan-lu.Isolation,screening and molecular biological Characterization of yeasts producing 4-hydroxy-2,5-dimethyl-3(2H)-furanone(HDMF)[J].Science and Technology of Food Industry,2010(2). |
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| Authors: | WANG Peng-xiao CHENG Lin-kun CHEN Cun-she LV Yue-gang SONG Huan-lu |
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| Affiliation: | WANG Peng-xiao,CHENG Lin-kun,CHEN Cun-she,LV Yue-gang,SONG Huan-lu(College of Chemistry , Environmental Engineering,Beijing Technology , Business University,Beijing 100048,China) |
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| Abstract: | Yeasts having the abilities for producing 4-hydroxy-2,5-dimethyl-3(2H)-furanone(HDMF) were screened from fruits such as pineapple and strawberry using D-fructose as carbon source. Spread plate method was used to separate yeasts strain from fruits,HPLC was used to determine the yield of HDMF by yeasts. 26S rDNA D1/D2 area sequential analysis and phylogenetic analysis were used to indentify the strains. 46 Yeasts were obtained,of which 5 had the abilities of producing HDMF using D-fructose;2 strains had much ... |
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| Keywords: | microorganism fermentation HDMF 26S rDNA |
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