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| 阴离子交换层析法分离纯化γ-PGA | |
| 引用本文： | 王浩,赵祥颖,田延军,乔君,刘建军.阴离子交换层析法分离纯化γ-PGA[J].食品工业科技,2012,33(11):253-255,263. |
| 作者姓名： | 王浩 赵祥颖 田延军 乔君 刘建军 |
| 作者单位： | 1. 山东轻工业学院食品与生物工程学院,山东济南,250353 2. 山东食品发酵工程重点实验室,山东济南,250013 3. 山东轻工业学院食品与生物工程学院,山东济南250353／山东食品发酵工程重点实验室,山东济南250013 |
| 摘 要： | 采用阴离子交换层析法,对γ-PGA进行分离纯化。通过阴离子树脂的静态吸附、动态实验,得出了上样的最佳pH8.0,上样量27.3mg/g树脂,洗脱液为0.7mol/L NaCl溶液,洗脱流速1.0mL/min,洗脱温度20℃。最终γ-PGA的纯度由86.7%提高到96.2%。 |
| 关 键 词： | γ-聚谷氨酸 静态吸附 分离纯化 |
Isolation and purification of γ-PGA by anion exchange column chromatography |
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| WANG Hao,ZHAO Xiang-ying,TIAN Yan-jun,QIAO Jun,LIU Jian-jun.Isolation and purification of γ-PGA by anion exchange column chromatography[J].Science and Technology of Food Industry,2012,33(11):253-255,263. | |
| Authors: | WANG Hao ZHAO Xiang-ying TIAN Yan-jun QIAO Jun LIU Jian-jun |
| Affiliation: | 1,2,*(1.College of Food and Biological Engineering,Shandong Polytechnic University,Jinan 250353,China; 2.Shandong Key Lab of Food and Fermentation Engineering,Jinan 250013,China) |
| Abstract: | γ-PGA was purified by anion exchange column chromatography.Through statical adsorption test and dynamic test of the anion resin,the optimum technological conditions were determined as follows:sample pH 8.0,sample quantity of resin 27.3mg/g,eluant 0.7 mol/L NaCl,eluting velocity 1.0mL/min,and eluting temperature 20℃.At last,the purity of γ-PGA was improved from 86.7% to 96.2%. |
| Keywords: | γ-Poly glutamic acid statical adsorption separation and purification |
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