一株芒果炭疽病拮抗菌抑菌活性的研究及其鉴定

从芒果表皮分离得到29株内生细菌,对峙培养法发现菌株B2-1对芒果炭疽病菌丝的生长具有较强的抑制作用。通过离体实验和活体实验对拮抗菌株B2-1抑菌活性进行了研究,离体实验结果表明,B2-1的发酵滤液、挥发性代谢产物以及非挥发性代谢产物都对芒果炭疽病菌丝的生长有很强的抑制作用,其中B2-1发酵液的非挥发性代谢产物对芒果炭疽病菌丝生长的抑制率达到了99.86%,抑菌效果显著。活体实验结果表明,B2-1的菌悬液和发酵液与对照相比显著降低了芒果炭疽病的发病率,提高了芒果的可食用果率。进一步对菌株B2-1进行鉴定,综合形态学观察和生理生化特征检测以及16S rDNA序列分析,B2-1菌株被鉴定为枯草芽孢杆菌。

Study on antibacterial activity and identification of an antagonistic bacterium for controlling anthracnose diseases of mango

Five antagonistic bacteria were obtained from 29 endosymbiotic bacteria.B2-1 strain was found to have the strongest antagonism to C.gloeosporioides.In vitro evaluation of antagonistic activity,whether the fermentation filtrate,volatile metabolites and non-volatile metabolites of B2-1 strain,showed a strong inhibition on the mycelia growth of C.gloeosporioides,the non-volatile metabolites of fermentation broth were able to inhibit the mycelial growth of the C.gloeosporioides by 99.86%.For further study,in vivo tests indicated that cell suspension and fermentation broth of bacteria B2-1 also significantly reduced the incidence of mango anthrax,thus showing a good potential for bio-controlling of anthracnose diseases.B2-1 was identified as bacillus subtilis by its morphological features,physiological-biochemical properties and 16S rDNA sequencing analysis.