| 响应面优化酶法提取青钱柳黄酮的工艺 |
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| 引用本文： | 柳旭光,杨洋,王峰,袁经权,缪剑华.响应面优化酶法提取青钱柳黄酮的工艺[J].食品工业科技,2012,33(5):250-253. |
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| 作者姓名： | 柳旭光 杨洋 王峰 袁经权 缪剑华 |
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| 作者单位： | 1. 广西大学生命科学与技术学院,广西南宁,530004
2. 广西药用植物园,广西南宁,530023 |
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| 基金项目： | 广西科技厅科技攻关项目(桂科攻07180063) |
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| 摘 要： | 研究了纤维素酶解法对青钱柳总黄酮的提取,考察了酶解浓度、酶解提取温度、酶解提取时间等单因素对青钱柳总黄酮提取得率的影响。在单因素实验基础上通过响应面分析优选出最佳的提取条件为:酶解时间为32.28min、温度为41℃、加酶量为0.57%,缓冲液pH为6.37,在此条件下青钱柳总黄酮的提取得率为9.227%。
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| 关 键 词： | 青钱柳 总黄酮 酶法提取 提取得率 响应面法 |
Optimization of total flavonoids extraction from Cyclocaraya paliurus by enzymatic hydrolysis using response surface methodology |
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| LIU Xu-guang,YANG Yang,WANG Feng,YUAN Jing-quan,MIAO Jian-hua.Optimization of total flavonoids extraction from Cyclocaraya paliurus by enzymatic hydrolysis using response surface methodology[J].Science and Technology of Food Industry,2012,33(5):250-253. |
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| Authors: | LIU Xu-guang YANG Yang WANG Feng YUAN Jing-quan MIAO Jian-hua |
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| Affiliation: | 2,*(1.Life Science and Technology College,Guangxi University,Nanning 530004,China;2.Guangxi Medicinal Herb Garden,Nanning 530023,China) |
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| Abstract: | The cellulose enzymatic-assisted extraction of total flavonoids from Cyclocarya paliurus by using response surface method which was on the basis of simple factor experiment has been studied.The optimum enzymatic-aided extraction conditions were:extraction time was 32.28min,temperature at 41℃ and cellulose enzyme amount was 0.57%,pH of extracting media was 6.37.Under these conditions,9.227% yield of total flavonoids was obtained. |
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| Keywords: | Cyclocaraya paliurus total flavonoids enzymatic extraction extraction rate response surface method |
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