山银花不同萃取部位抗炎、抗氧化体外活性评价及化学成分分析

本研究采用脂多糖（LPS）诱导的细胞炎症模型和H₂O₂诱导的细胞氧化应激模型，对山银花不同萃取部位进行抗炎、抗氧化活性评价，并通过超高压液相色谱串联四极杆飞行时间质谱（UPLC-Q-TOF-MS）分析具有显著活性的萃取部位的化学成分。结果表明，山银花甲醇提取物及其各萃取部位（50 μg/mL）均有不同程度的抗炎、抗氧化活性。其中水饱和正丁醇萃取部位的抗炎活性最强，对RAW264.7细胞内NO、TNF-α、IL-6等炎症因子的抑制率分别达66.40%、13.14%、79.66%，初步鉴定该部位中的17个有机酸类和皂苷类成分；乙酸乙酯萃取部位的抗氧化能力最强，可对RIN细胞中抗氧化酶SOD、GSH、CAT三种抗氧化因子的活性分别提高2.51、4.40、8.89倍，初步鉴定该部位中的12个有机酸类成分。因此，有机酸和皂苷类成分可能是山银花具抗炎、抗氧化活性的主要物质基础。本文为进一步阐明山银花具生物活性的物质基础及开发利用提供数据支撑。

Evaluation of in Vitro Anti-inflammatory and Antioxidant Activities and Analysis of Chemical Components in Different Extraction Parts of Lonicerae Flos

The aim of this study is to elucidate the material basis of Lonicerae Flos biological activity and provide a theoretical basis for its development and utilization,lipopolysaccharide(LPS)-induced cell inflammation model and H2O2-induced oxidative stress model were used to evaluate the anti-inflammatory and antioxidant activities of different extraction parts of Lonicerae Flos.The chemical constituents of the extractions with significant activities were analyzed by ultra-high pressure liquid chromatography tandem quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).The results showed that the methanol extracts of Lonicerae Flos(50μg/mL)have different levels of anti-oxidation and antiinflammatory effects.The extraction site of saturated n-butanol effectively inhibited the inflammatory factors(NO、TNF-αand IL-6)of RAW264.7 cells,suggesting it has the strongest anti-inflammatory activity.The inhibition rates of inflammatory factors of NO,TNF-αand IL-6 were 66.40%,13.14%and 79.66%respectively.16 compounds in the watersaturated n-butanol extractive site were identified,and which mainly consisted of organic acids.The ethyl acetate extractive site has the strongest antioxidant ability.The antioxidant enzyme activity in RIN cells had increased about 2.51(SOD),4.40(GSH),8.89(CAT)times respectively.After preliminary identification,12 compounds,including organic acids and saponins in the ethyl acetate site,were the main compound.Therefore,organic acids and saponins were the main chemical components of Lonicerae Flos,which had the antioxidant and anti-inflammatory activities.This article provides data support for further elucidating the material basis and development and utilization of Lonicerae Flos.