10种广东药食两用植物的抗氧化和抗增殖活性评价

测定了10种广东药食两用植物的总黄酮含量（AlCl₃-NaNO₂法）和总多酚含量（Folin-Ciocalteu法），评价了其抗氧化活性（DPPH自由基清除活性和羟自由基清除活性）和抗小鼠结肠癌MC-38细胞增殖活性（CCK-8试剂盒法），分析了活性物质功效与其含量之间的相关性。结果表明:在10种广东药食两用植物中，总黄酮含量最高的为高良姜（85.76 ± 3.25 μmol CE/g DW），含量最低的为沙姜（0.80 ± 0.05 μmol CE/g DW）和龙眼肉（0.69 ± 0.03 μmol CE/g DW）。总多酚含量最高的为高良姜（226.26 ± 9.17 μmol GAE/g DW），含量最低的为沙姜（7.52 ± 0.67 μmol GAE/g DW）。清除DPPH自由基活性最强的为高良姜，活性最弱的为沙姜。清除羟自由基活性最强的为沙姜、益智仁和肉豆蔻（三者无显著性差异），活性最弱的为陈皮。抗MC-38细胞增殖活性最强的为陈皮和高良姜，活性最弱的为龙眼肉。DPPH自由基清除活性与其总黄酮和总多酚含量之间均有显著正相关性（P<0.05）；抗MC-38细胞增殖活性与其总黄酮和总多酚含量之间均有极显著正相关性（P<0.01）；羟自由基清除活性与其总黄酮和总多酚含量之间相关性均不显著。药食两用植物的活性功效虽有剂量效应，但还受到其成分种类、协同作用等影响。

Evaluation of Antioxidant and Antiproliferation Activities of 10 Kinds of Guangdong Medicinal and Edible Plant

The flavonoids content(AlCl3-NaNO2 method)and total polyphenol contents(Folin-Ciocalteu method)of 10 kinds of Guangdong medicinal and edible plant extracts were determinated.The antioxidant activity(DPPH radical scavenging activity and hydroxyl radical scavenging activity)and the antiproliferation activity of mice colorectal cancer MC-38 cell(CCK-8 kit method)were evaluated.The correlation between the activity of active substance and its contents were analyzed.The results showed that the highest content of total flavonoids was galangal(85.76±3.25μmol CE/g DW)of 10 Guangdong medicinal and edible plants,and the lowest content of total flavonoids were Kaempferia(0.80±0.05μmol CE/g DW)and longan(0.69±0.03μmol CE/g DW).The highest content of total polyphenol(226.26±9.17μmol GAE/g DW),and the lowest content of total polyphenol was Kaempferia(7.52±0.67μmol GAE/g DW).The DPPH free radical scavenging activity of the strongest for galangal,the weakest activity for Kaempferia.The activity of scavenging hydroxyl free radical was strongest in Kaempferia,Alpinia oxyphylla and nutmeg(there was no significant difference among them),and the activity was weakest in orange peel.The anti-MC-38 cell proliferation activity was strongest in orange peel and galangal,and weakest in longan.DPPH radical scavenging activity was significantly correlated with the content of total flavonoids and total polyphenols(P<0.05).The correlation between Anti-MC-38 cell proliferation activity and the content of total flavonoids and total polyphenol was very significant(P<0.01).There was no significant correlation between the scavenging activity of hydroxyl radical and the content of total flavonoids and total polyphenols.Although the active efficacy of water extract of edible and medicinal plants had dose effect,it was also affected by the kinds and synergistic effects of its components.