超声波辅助提取玉米花丝多酚工艺优化及其抑菌活性研究

本文以玉米花丝多酚为研究对象，在单因素实验的基础上，利用响应面优化超声波辅助提取玉米花丝多酚工艺，考察超声波频率、超声波功率、超声波温度和超声波时间对玉米花丝多酚提取量的影响，同时探究玉米花丝多酚对大肠杆菌和金黄色葡萄球菌的抑菌活性。结果表明:在液料比30: 1、乙醇浓度60%、超声波频率40 kHz、超声波功率350 W、超声波温度56 ℃、超声波时间45 min时，玉米花丝多酚提取量为（13.31±0.12）mg/g；抑菌活性试验表明:不同浓度玉米花丝多酚对大肠杆菌和金黄色葡萄球菌均有一定的抑制效果，其最小抑菌浓度分别为2.5 mg/mL和2 mg/mL。

Optimization of Ultrasonic-assisted Extraction of Corn Silk Polyphenols and Its Antibacterial Activity

In this experiment, corn silk polyphenols were taken as the research object. Based on the single factor experiment, response surface methodology was used to optimize the ultrasonic-assisted extraction process of corn silk polyphenols. The effects of ultrasonic frequency, ultrasonic power, ultrasonic temperature and ultrasonic time on the extraction rate of corn silk polyphenols were investigated, and the antibacterial activity of corn silk polyphenols against Escherichia coli and Staphylococcus aureus was also explored. The results showed that under the conditions of ethanol concentration 60%, liquid-solid ratio 30:1, ultrasonic frequency 40 kHz, ultrasonic power 350 W, ultrasonic temperature 56 ℃ and ultrasonic time 45 min, the extraction rate of corn silkpolyphenols were (13.31±0.12) mg/g; The antibacterial activity test showed that corn silk polyphenolswith different concentrations had certain inhibitory effect on Escherichia coli and Staphylococcus aureus, and the minimum inhibitory concentrations were 2.5 mg/mL and 2 mg/mL respectively.